Alpha Synuclein S87N Mutant Pre-formed Fibrils

Synonyms

MAPT, 2N4R, Tau40 neurofibrillary tangle protein, paired-helical filament, PHFs, SNCA, NACP, PARK1, asyn, alpha-synuclein, pre-formed fibril, PFFs, mixed fibrils

Background

Human alpha-synuclein S87N mutant (HuS87N) has Ser87 mutated to the equivalent mouse residue Asn87, effectively making it a human-mouse chimeric protein. Despite sequence differences at only seven residues, or 5% of the total 140 amino acids, the aggregation rate of wild-type mouse α-syn (MsWT) is faster than wild-type human α-syn (HuWT) in vitro. In wild-type mouse models, MsWT fibrils are more efficient than HuWT fibrils at inducing endogenous mouse α-syn pathology. A53T or S87N substitutions in human α-syn substantially accelerate fibrilization rates in vitro. Chimeric HuS87N fibrils show enhanced induction of α-syn pathology greater than both HuWT and MsWT fibrils in mice neuron cultures. Therefore, HuS87N is a good construct for inducing robust endogenous α-syn seeding and pathology in wild-type mice/cultures.

Description

Human Recombinant Alpha Synuclein S87N Mutant Pre-formed Fibrils

SWISS

P37840-1

Accession Number

NP_000336.1

Gene ID

6622

Amino Acid Sequence

MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA

Protein Length

140 aa

Applications

WB, SDS PAGE, In vitro assay

Research Areas

Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Tangles & Tau, Multiple System Atrophy

Concentration

Lot/batch specific. See included datasheet.

Nature

Recombinant

Storage Buffer

1X PBS pH 7.4

Purity

>95%

Purification

Ion-exchange Purified

Storage

Stored at -80°C

Shipping

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

Notes

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Species	Human
Tag	Tag Free
Expression Systems	E.coli
Keywords	Preformed fibrils