CHAT Polyclonal Antibody

Product Information

It targets CHAT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively. This antibody can recognize both of pCHAT and cCHAT.

Synonyms

CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2

Full Name

Choline acetyltransferase

Tested Reactivity

Human, mouse, rat

Type

Antibody

Class

Polyclonal

Immunogen

Peptide

Calculated Molecular Weight

83 kDa

Observed Molecular Weight

47-50 kDa, 66-70 kDa

GenBank Accession Number

NM_020549

Gene ID

1103

Gene Symbol

CHAT

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive WB Detected

Mouse brain tissue, mouse placenta tissue, human placenta tissue, Jurkat cells, rat brain tissue

Positive IP Detected

Rat brain tissue

Positive IHC Detected

Mouse cerebellum tissue, mouse brain tissue, rat brain tissue, human lung cancer tissue

Positive IF Detected

Mouse brain tissue

Target	CHAT
Cell Type	Motor neurons
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, FC, ELISA
Reactivity	Human, mouse, rat
Conjugate	Unconjugated
Keywords	Motor neurons; Rabbit; IgG; WB, IP, IHC, IF, FC, ELISA