EZH2 Polyclonal Antibody

Product Information

The immunogen of EZH2 Fusion Protein expressed in E. coli.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa).

Full Name

Enhancer of zeste homolog 2 (Drosophila)

Synonyms

Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

Tested Reactivity

Human, mouse, rat

Cited Reactivity

Human, mouse, rat, pig

Class

Polyclonal

Type

Antibody

Immunogen

EZH2 fusion protein

Calculated Molecular Weight

751 aa, 86 kDa

Observed Molecular Weight

90-102 kDa

Gene ID

2146

GenBank Accession Number

BC010858

Gene Symbol

EZH2

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive IP Detected

HepG2 cells

Positive IF Detected

HepG2 cells

Positive FC Detected

HepG2 cells

Target	EZH2
Cell Type	Oligodendrocytes
Host	Rabbit
Isotype	IgG
Applications	WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA
Reactivity	Human, Mouse, Rat, Pig
Conjugate	Unconjugated
Keywords	Oligodendrocytes; Rabbit; IgG; WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA