Galc Polyclonal Antibody

Product Information

It targets Galc in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Background Information

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).

Full Name

Galactosylceramidase

Synonyms

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

Tested Reactivity

Human, mouse, rat

Cited Reactivity

Human, mouse, zebrafish

Class

Polyclonal

Type

Antibody

Immunogen

Galc fusion protein

Calculated Molecular Weight

77 kDa

Observed Molecular Weight

80 kDa, 30 kDa, 50 kDa

Gene ID

14420

GenBank Accession Number

BC086671

Gene Symbol

Galc

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive WB Detected

A375 cells, A549 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

Positive IP Detected

NIH/3T3 cells

Positive IHC Detected

Human gliomas tissue

Target	GALC
Cell Type	Oligodendrocytes
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, ELISA
Reactivity	Human, Mouse, Rat, Zebrafish
Conjugate	Unconjugated
Keywords	Oligodendrocytes; Rabbit; IgG; WB, IP, IHC, IF, ELISA