GFAP Polyclonal Antibody

Product Information

It targets GFAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Background Information

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. Tissue specificity GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease

Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations
present in affected individuals are thought to be gain-of-function.

Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular
processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various
neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916),
which means that they differ in molecular size. Isoform expression varies
during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP
protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Synonyms

GFAP

Full Name

Glial fibrillary acidic protein

Tested Reactivity

Human, mouse, rat

Cited Reactivity

Human, mouse, rat, goat, hamster, pig, zebrafish

Class

Polyclonal

Type

Antibody

Immunogen

GFAP fusion protein

Calculated Molecular Weight

432 aa, 50 kDa

Observed Molecular Weight

45-50 kDa

Gene ID

2670

GenBank Accession Number

BC013596

Gene Symbol

GFAP

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive WB Detected

Mouse brain tissue, U-251 cells

Positive IF Detected

Rat brain tissue

Target	GFAP
Cell Type	Radial glial cells, glial progenitors
Host	Rabbit
Isotype	IgG
Applications	WB, IHC, IF, ELISA
Reactivity	Human, mouse, rat, goat, hamster, pig, zebrafish
Conjugate	Unconjugated
Keywords	Radial glial cells, glial progenitors; Rabbit; IgG; WB, IHC, IF, ELISA