GLAST Polyclonal Antibody

Product Information

The immunogen of GLAST Fusion Protein expressed in E. coli.

Background Information

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3.

Synonyms

EA6, EAAT1, GLAST, GLAST 1, GLAST1, SLC1A3

Full Name

Solute carrier family 1 (glial high affinity glutamate transporter), member 3

Tested Reactivity

Human, mouse, rat

Cited Reactivity

Human, mouse, rat, cow

Type

Antibody

Class

Polyclonal

Immunogen

GLAST fusion protein

Calculated Molecular Weight

542 aa, 60 kDa

Observed Molecular Weight

50-55 kDa, 90-100 kDa

GenBank Accession Number

BC037310

Gene ID

6507

Gene Symbol

SLC1A3

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive WB Detected

Neuro-2a cells, C6 cells, mouse brain tissue

Positive IP Detected

Mouse brain tissue

Positive IHC Detected

Human brain tissue, mouse brain tissue

Target	GLAST
Cell Type	Type 2 astrocytes
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, ELISA
Reactivity	Human, mouse, rat, cow
Conjugate	Unconjugated
Keywords	Type 2 astrocytes; Rabbit; IgG; WB, IP, IHC, IF, ELISA