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Human Alpha-Synuclein Protein, His Tag

CAT#: IVD-P004

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Overview Specification

Species	Human
Tag	His
Expression Systems	E.coli
Keywords	In vitro diagnostic proteins

Synonyms	SNCA, NACP, PARK1, alpha-Synuclein
Background	Neuronal protein plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for enhancing ATP-induced exocytosis. It also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SREs (Soluble NSF Attachment Protein REceptors) at the presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DJC5. This chaperone activity is important to sustain normal SRE-complex assembly during aging. It also plays a role in regulating dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
Source	Human Alpha-Synuclein
SWISS	P37840-1
Theoretical Molecular Weight	16.3 kDa
Formulation	Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0 with trehalose as protectant.
Reconstitution	Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Molecular Characterization	This protein carries a polyhistidine tag at the C-terminus. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin	Less than 1.0 EU per μg by the LAL method.
Sterility	The sterility testing was performed by membrane filtration method.
Purity	> 90% as determined by SDS-PAGE.
Storage	For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Notes	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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