Alfa Cytology/Brain Tumors

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Human APP/Abeta40 Protein, His Tag

CAT#: IVD-P001

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Overview Specification
Species Human
Tag His
Expression Systems E.coli
Keywords In vitro diagnostic proteins
Synonyms ABPP, APPI, Amyloid-beta A4 protein
Background Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. It has three predominant splice variants: APP695, APP751, and APP770. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C99, which is subsequently cleaved by γ-secretase to release Aβ.
Source Human Abeta40
SWISS P05067-1
Theoretical Molecular Weight 6.3 kDa
Formulation Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH 7.4 with trehalose as protectant.
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Molecular Characterization This protein carries a polyhistidine tag at the C-terminus. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Less than 1.0 EU per μg by the LAL method.
Purity > 95% as determined by SDS-PAGE.
Storage For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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