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Human N-cadherin ELISA Kit

CAT#: NRE-K010

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Overview Specification

Sample Type	Serum, plasma, cell culture supernatants
Sensitivity	5.6 pg/mL
Range	46.88-3,000 pg/mL
Reactivity	Human
Keywords	Serum, plasma, cell culture supernatants; Human

Product Information	It is a solid-phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The N-caderin ELISA kit is to be used to detect and quantify protein levels of endogenous N-caderin. The assay recognizes human N-caderin. An antibody specific for N-caderin has been pre-coated onto the microwells. The N-caderin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human N-caderin is added to detect the captured human N-caderin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Background Information	Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130 kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes. N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity. Soluble N-cadherin (sN-CAD) is found in biological fluids, including serum, seminal fluid samples, and urine. It has been shown that significantly higher amounts of serum sN-CAD are present in cancer patients than in persons with no evidence of disease.
Synonyms	Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin
Tests	1 x 96 well plate
Assay Type	Sandwich
Tested Applications	Sandwich ELISA
Gene ID	1000
Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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