NF-L Polyclonal Antibody

Product Information

It targets NF-L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L

Full Name

Neurofilament, light polypeptide

Tested Reactivity

Human, mouse, rat

Type

Antibody

Class

Polyclonal

Immunogen

NF-L fusion protein

Calculated Molecular Weight

543 aa, 62 kDa

Observed Molecular Weight

65-68 kDa, 140-160 kDa

GenBank Accession Number

BC039237

Gene ID

4747

Gene Symbol

NEFL

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive WB Detected

SH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells

Positive IP Detected

Mouse brain tissue

Positive IHC Detected

Mouse brain tissue

Positive IF Detected

SH-SY5Y cells

Positive FC Detected

SH-SY5Y cells

Target	NF-L
Cell Type	Dopaminergic, glutamatergic, GABAergic neurons
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, FC, ELISA
Reactivity	Human, mouse, rat
Conjugate	Unconjugated
Keywords	Dopaminergic, glutamatergic, GABAergic neurons; Rabbit; IgG; WB, IP, IHC, IF, FC, ELISA