NF-M Polyclonal Antibody

Product Information

The immunogen of NF-M Fusion Protein expressed in E. coli.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognizes endogenous NF-M protein.

Synonyms

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

Full Name

Neurofilament, medium polypeptide

Tested Reactivity

Human, rat, mouse

Type

Antibody

Class

Polyclonal

Immunogen

NF-M fusion protein

Calculated Molecular Weight

102 kDa

Observed Molecular Weight

140 kDa

GenBank Accession Number

BC002421

Gene ID

4741

Gene Symbol

NEFM

Form

Liquid

Purification Method

Antigen affinity purification

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Positive WB Detected

Mouse brain tissue, rat brain tissue

Positive IHC Detected

Mouse cerebellum tissue, human brain tissue, human colon tissue, mouse brain tissue

Positive IF Detected

Mouse brain tissue

Target	NF-M
Cell Type	Dopaminergic, glutamatergic, GABAergic neurons
Host	Rabbit
Isotype	IgG
Applications	WB, IHC, IF, ELISA
Reactivity	Human, mouse, rat, canine
Conjugate	Unconjugated
Keywords	Dopaminergic, glutamatergic, GABAergic neurons; Rabbit; IgG; WB, IHC, IF, ELISA