Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)

Synonyms

MAPT, intracellular neurofibrillary tangles, NFTs, paired helical filaments, PHFs, 2N4R

Background

Brain-specific tau isoforms vary in the number of N-terminal inserts and C- C-terminal repeat domains due to alternative splicing of exons; the 2N4R isoform of tau is expressed in the adult brain yet is absent from the fetal brain. Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer’s Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils. Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies. Sf9/Baculovirus-expressed Tau 2N4R Pre-Formed Fibrils are generated without heparin.

Description

Human Recombinant Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)

SWISS

P10636-8

Amino Acid Sequence

MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

Applications

WB, SDS PAGE, In vitro assay

Research Areas

Alzheimer's Disease, Neurodegeneration, Neuroscience, Tangles & Tau

Concentration

Lot/batch specific. See included datasheet.

Nature

Recombinant

Storage Buffer

1X PBS pH 7.4

Purity

>95%

Purification

Ion-exchange Purified

Storage

Stored at -80°C

Shipping

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

Notes

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Species	Human
Tag	Tag Free
Expression Systems	Baculovirus (Sf9)
Keywords	Preformed fibrils