In order to identify early diagnosis strategies, preventive measures, and effective interventions, a thorough understanding of the specific cellular and molecular mechanisms of PC development and progression is required. To conduct research in pancreatic cancer (PC), established cancer cell lines remain powerful tools. Here, we offer PC cell analysis and evaluation services to help researchers and professionals select the cell line that best fits their particular research needs.
Phenotype and genotype of PC Cell Lines
As a valuable resource, the established cell lines are widely utilized. To date, there is a range of PC cell lines have been established. For different research needs, cell lines should be carefully evaluated before selection and attention should be paid to cell line identification, including in vivo and in vitro growth characteristics, phenotypic characteristics (e.g., adhesion, invasion, migration, tumorigenesis, and angiogenic potential), and genotypic status of commonly altered genes.
The service offering at Alfa Oncology
Our PC cell analysis and evaluation platform is supported by state-of-the-art infrastructure as well as market-leading technology, such as plate readers, liquid handling instruments, and automated live-cell imaging systems. We are now offering PC cell analysis and evaluation services, including but not limited to the following list:
- PC cell size and morphology determination
- PC cell proliferation and viability assays (such as spheroid formation assay)
- PC cell adhesion
Cell adhesion depends on the interaction of cell surface molecules with extracellular matrix components (such as fibronectin, collagens I and IV, and laminin). The high metastatic potential of PC highlights the importance of detecting cancer cell adhesion properties.
- Cell migration and invasion assays ( such as analyzed through scratch assay)
Cell migration is also an important component of PC spread, and studying the ability of PC cells to migrate is necessary to gain insight into the biological processes that mediate metastasis. Moreover, PC is by nature highly aggressive, with almost all patients presenting with metastasis at diagnosis, thus invasion is another important phenotype of PC cells. Notably, migration analysis monitors cell movement, while invasion analysis monitors cell movement through specific substrates.
- Angiogenic potential
Angiogenesis is the process by which cancer cells induce endothelial cell proliferation and leading to the subsequent formation of new blood vessels, which is a typical feature of tumor growth and metastasis. To evaluate the angiogenic potential of PC cell lines, the expression of pro-angiogenic chemokines, cytokines, and enzymes have been applied.
Tumorigenicity demonstrates the ability or propensity of a cancer cell line to produce tumors in vivo. Tumor formation, including frequency of occurrence and growth rate, is generally measured starting after PC cell injection in immunocompromised mice, followed by measurement of tumor volume and/or mass at necropsy. These parameters mentioned above have been commonly used to estimate tumorigenicity.
There are many high-frequency gene mutations in PC, such as KRAS(93%), TP53 (72%), CDKN2A (30%), SMAD4 (32%), etc.
- PC cell cycle and growth kinetics analysis
- PC cell metabolism analysis
- Quality control of PC cells
Key benefits of our services
- Deliver efficient and robust results for cell- and biochemical-based assays.
- Real-time data provides comprehensive information on cellular responses over long periods of time.
- Support detailed quantitative analysis while providing powerful insight into cellular behavior over time.
- High throughput and reliable analysis.
Alfa Oncology is a leading provider of PC drug development services. Our team of scientists has expertise in basic science, with personal expertise in immunology, drug development, genomics, and imaging to develop new PC therapies. We are providing a range of services around PC, including important parts such as basic research, drug development, and diagnosis of PC. In order to get more details about our services, please contact us. We are glad to work with you!
- Deer, E. L., González-Hernández, J., Coursen, J. D., Shea, J. E., Ngatia, J., Scaife, C. L., ... & Mulvihill, S. J. (2010). Phenotype and genotype of pancreatic cancer cell lines. Pancreas, 39(4), 425.
- Di Federico, A., Tateo, V., Parisi, C., Formica, F., Carloni, R., Frega, G., ... & Brandi, G. (2021). Hacking Pancreatic Cancer: Present and Future of Personalized Medicine. Pharmaceuticals, 14(7), 677.