Pancreatic ductal adenocarcinoma (PDAC) remains one of the most horrific and lethal malignancies in the world, with a 5-year survival rate of less than 10%. PDAC is often discovered at an advanced stage and is highly resistant to existing therapies, in part due to the extremely high incidence of distant metastasis (>80%) at initial diagnosis. Transcriptomics is a discipline that studies the transcription and transcriptional regulation of all genes in cells at an overall level. The research objects of transcriptomics include mRNA and non-coding RNA. The new generation of high-throughput sequencing technology can comprehensively and quickly obtain the sequence information and expression information of almost all transcripts of a specific cell or tissue in a certain state, thereby accurately analyzing gene expression differences, gene structural variations, and interactions between genes. Single-cell transcriptomic profiling of PDAC primary tumors has been systematically investigated, revealing that the immune and stromal landscapes in each patient are highly heterogeneous, and that cytotoxic T cells with exhausted gene expression patterns might contribute to the immunosuppressive TME.
Fig. 1. Single-cell transcriptomics is used to create reference maps of healthy human tissues, organs, and systems at single-cell resolution. (Aldridge S, et al., 2020)
Services We Offer
Transcriptomics can study the expression levels of biological genes in specific time and space, thereby revealing its intrinsic mechanism and providing new ideas for in-depth research on pancreatic cancer. Alfa Cytology can provide you with comprehensive RNA sequencing services, professional and customized bioinformatics analysis services based on sequencing data.
- Eukaryotic Transcriptome Sequencing
Transcriptome sequencing is a high-throughput sequencing of the mRNA produced by a certain species or a specific cell in a certain functional state. It can not only provide quantitative analysis to detect differences in gene expression levels, but also provide structural analysis to discover rare transcripts. Alternative splice sites, gene fusions, etc. can be accurately identified without relying on a reference genome.
- RNA-Seq
RNA-Seq, which performs high-throughput sequencing of mRNA produced by a certain species or a specific cell in a certain functional state, can provide quantitative analysis and detect differences in gene expression levels.
- UMI Small RNA Sequencing
Specific molecular tags (UMI) are introduced during library construction and combined with high-throughput sequencing to study the sequence information of 18-30 nt small RNA fragments in tissues under specific spatiotemporal conditions to achieve accurate quantification. By comparing with the database, it can realize the identification of small RNA sequences, target gene analysis and functional analysis, as well as the analysis and research needs of various small RNA types including miRNA, siRNA, piRNA, etc.
- Long non-coding RNA Sequencing
Conduct sequencing research on large RNAs such as long non-coding RNA, mRNA, and circular RNA to quickly, comprehensively and accurately obtain all large RNA transcript data information related to specific biological processes (such as development, disease, etc.).
- Circular RNA Sequencing
circRNA is a special type of non-coding RNA molecule. The circRNA molecules have a closed ring structure and are not affected by RNA exonucleases. Their expression is more stable and difficult to degrade. It can be used in the construction of species circRNA expression profiles, the development of circRNA disease biomarkers, and the study of circRNA action mechanisms.
- Full-length Transcriptome
Full-length transcriptome sequencing technology can directly obtain full-length transcript information without assembly, ensuring the accuracy of transcriptome sequencing results to a greater extent and supplementing the gene annotation results of annotated genomes.
Alfa Cytology is committed to providing customers with one-stop pancreatic cancer basic research services. If you are interested in our pancreatic cancer transcriptome sequencing services, please contact us for more details.
Reference
- Aldridge S, Teichmann SA. Single cell transcriptomics comes of age. Nat Commun. 2020 Aug 27;11(1):4307.