Pancreatic ductal adenocarcinoma is one of the most common malignant tumors. Early diagnosis and treatment are very difficult, and the mortality and recurrence rates are high. Chronic pancreatitis is an important risk factor for pancreatic cancer. Therefore, studying the mechanism of transformation from pancreatitis to pancreatic cancer is very necessary for the early diagnosis and treatment of pancreatic cancer. Epigenomics studies epigenetic modifications at the genome level and studies the modification information of non-DNA sequence changes within the scope of "omics". For the genome, not only the DNA sequence contains genetic information, but its modifications can also record genetic information. In recent years, more and more studies have shown that epigenetic modifications play an important role in the transformation of pancreatic cancer and can provide potentially effective targets for the diagnosis and treatment of pancreatic cancer.
Fig. 1. Pancreatic ductal adenocarcinoma of normal pancreas, pancreatic intraepithelial neoplasia and metastasis. (Wang SS, et al., 2021)
Services We Offer
Alfa Cytology provides epiomics services, including two standard products: whole-genome Bisulfite methylation sequencing and ChIP-seq, as well as multiple customized products such as target region capture methylation sequencing, ATAC-Seq, and OxBS.
- Whole-genome Bisulfite methylation sequencing
It is the gold standard in DNA methylation research. It analyzes the methylation status of the entire genome through a combination of Bisulfite treatment and whole-genome DNA sequencing. It has single-base resolution and can accurately assess the methylation level of a single C base with wide coverage. It can construct a fine methylation map, establish an epigenetic research database, and provide a reference map for subsequent large-scale analysis of methylation differences between different samples.
- ChIP-Seq
Chromatin immunoprecipitation (ChIP) is a classic experimental method to study the interaction between proteins and DNA in an in vivo environment. It is widely used in related fields such as histone modification and gene regulation of specific transcription factors.
- ATAC-Seq
Through the modification of transposase with extremely high activity, the open regions of chromatin structure are captured and sequenced. Real-time whole-genome active regulatory sequence information can be obtained with only a small number of cells. It is widely used in studies such as transcription factor binding analysis, nucleosome positioning, and distribution of active regulatory elements. It has broad application prospects in the field of epigenetic mechanism research.
Alfa Cytology is committed to providing customers with one-stop basic research services on pancreatic cancer. If you are interested in our pancreatic cancer epigenetic research services, please contact us for more details.
Reference
- Wang SS, et al. Epigenetic Alterations in Pancreatic Cancer Metastasis. Biomolecules. 2021 Jul 22;11(8):1082.