Search
Search
Figure 1. Process of cancer cell development.
Cell proliferation is a series of processes in which cells divide through complex reactions such as DNA replication, RNA transcription, and protein synthesis in response to cycle-regulating factors. Cell proliferation is an important life feature of living organisms, and cell proliferation by division is also the basis for the growth, development, reproduction, and inheritance of living organisms. The assay of cell proliferation capacity is the most common method to assess cell activity, genotoxicity, and the effect of antitumor drugs, etc. It is widely used in brain oncology and brain tumor drug screening.
Cellular activity and proliferation assays include both direct assays of DNA synthesis in cells and assays of cellular metabolic activity. Alfa Cytology has built an easy-to-operate, sensitive, and specific assay solution based on our excellent project experience, which can be flexibly selected according to customers' experimental needs. In addition, we also offer brain tumor cell proliferation inhibitor screening services.
We can monitor brain tumor cells and cell division by assessing DNA synthesis, monitoring mitotic stages, or tracking population ploidy.
| Project | Content | Results | |
| Cell culture and processing | Cell culture and drug treatment |
|
|
| MTT assay | It mainly reflects the energy metabolism of cells and is a simple and accurate method to detect cell proliferation viability. | Detection of cellular metabolic activity | |
| WTS-1 assay | It is more stable than XTT and MTS, which makes the experimental results more stable. | ||
| CCK8 assay | It is a rapid and highly sensitive assay based on WST-8 that is widely used for cell proliferation and cytotoxicity. | ||
| CTL assay | A method for the quantitative determination of live cell numbers by measuring intracellular ATP content by chemiluminescence with ultra-high sensitivity and ultra-wide linear range. | ||
| CFDA SE assay | It is a green fluorescent probe widely used in cell proliferation and tracing studies. It does not have fluorescent properties itself, but can permeate the cell membrane and is catalytically decomposed to produce CFSE after freely entering the cell, which has high fluorescence activity to label proteins. | Detection of DNA synthesis in cells | |
| EdU assay | A simple, rapid, and highly sensitive assay that directly measures DNA synthesis and can effectively detect the percentage of cells in the S-phase. | ||
| LDH release assay | Quantitative analysis of cytotoxicity can be achieved by detecting the activity of LDH released into the culture medium from cells with ruptured plasma membranes. | Detection of cellular LDH release | |
We offer a comprehensive anti-brain tumor drug screening platform, which includes antibody drug discovery, and in vitro and in vivo activity analysis services. As part of our in vitro brain oncology services, we have built a cost-effective human brain tumor cell line assay system that will provide you with a combination panel of multiple human tumor cell lines as well as high-throughput screening (HTS) services.
Alfa Cytology' technical service staff has the skills and experience to efficiently and accurately perform cell proliferation inhibition analysis services for brain tumors, ensuring accurate and reliable experimental data. Please contact our staff to learn more about our cell proliferation assay technology services.
Brain Tumors
Alfa Cytology has been committed to providing comprehensive services and products to our brain tumor research customers worldwide through our global network of operations.
Subscribe to Our Newsletter
Copyright © 2024 Alfa Cytology. All rights reserved.