Stable Cell Line Construction Services for Brain Tumors

High-quality stable cell lines play a very important role in brain tumor biology research, including gene function studies, recombinant antibodies, and drug development. Stable expression cell lines refer to cell lines constructed based on a cell line that consistently overexpresses or interferes with a specific gene. The basic principle is that exogenous DNA is cloned into a vector with a certain resistance, the vector is transfected into the host cell and integrated into the host chromosome, and the vector is screened with the resistance marker contained in the vector.

Our stable cell line construction services for brain tumors

Lentiviral infection-drug screening is a widely used method for constructing stable cell lines with efficient integration and a wide range of target cells. Alfa Cytology screens target cells for resistance markers on a transient transfection basis. Alternatively, high transfection efficiency viruses are used to select the appropriate drugs for cell passaging according to the resistance markers contained in the different gene vectors. This results in cell lines that can stably express the target gene in a short period.

• Hybrid clonal cell lines. Gene transfection followed by direct screening with resistance drugs. The screened cells express both the resistance gene and the target gene but contain a variety of different cell clones. The location of target gene integration and expression varies between clones. It has the advantage of relatively rapid screening and low cost.
• Monoclonal cell lines. A cell line obtained from the amplification of a single cell containing a stably integrated exogenous fragment. The expression of the target gene at the site of integration is highly uniform for each cell, but some phenotypes may be lost. Monoclonal cell screens are often recommended for cell lines that require cell sublocalization experiments, are difficult to infect, and have low expression rates.

Services flow

Some of the stable cell lines that have been constructed

Technical advantages

• Excellent seed cells. Low generation and no contamination.
• Stable production process. Strict screening and target gene expression verification system.
• No loss of cell viability. Rigorous evaluation of cell proliferation viability for 5 consecutive generations after line establishment.
• Cell line quality assurance. RT-qPCR validation to ensure expression level.

Applications

• A long-term study of gene function in target cells, we can reduce the cost of frequent transfection or virus packaging by establishing stable cell lines to facilitate experimental studies.
• Some proteins have extremely long half-lives, and transient RNA can only interfere with expression and cannot remove the already expressed target protein, we can achieve better gene interference effects by building stable cell lines.
• Transient will introduce extremely high copy number expression, leading to inaccurate experimental results due to human factors, we build stable cell lines that can help to screen cells with appropriate copy numbers for experimental studies.
• Those that require an inducible expression system are mainly some lethal genes or those that require spatiotemporal expression. Those that need to use cells for animal experiments, such as nude mice tumorigenesis, etc. We can establish stable cell lines for all of them.

Alfa Cytology has rich experience in constructing and screening stable cell lines for brain tumors. We can screen high-quality stable cell lines for our customers, and you can select the appropriate cell lines from our cell bank or provide their cell lines. Please get in touch with our staff to discuss your project requirements and get more information about our brain tumor stable cell line construction and screening services.