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Primary Immune Cell Analysis Services for Brain Tumors

Primary Immune Cell Analysis Services for Brain Tumors

Excessive inhibition or activation of immune cells is one of the reasons for the occurrence and development of brain tumors and some autoimmune diseases. Primary immune cell stimulation assay is an in vitro experimental method to screen out functionally active antibody molecules by evaluating antibodies' activating or inhibiting effect on immune cells isolated in vivo. The primary immune cell assay provides a more realistic response to the activity of antibody drugs in vivo. It helps researchers to more accurately screen for highly active antibody drug molecules during the R&D phase.

We provide primary immune cell analysis services for brain tumors

Alfa Cytology can provide primary immune cells from a wide range of stimuli and different species to help our clients assess the in vitro activity of antibodies by detecting immune cell proliferation, single or multiple cytokine releases, or the expression of immune cell surface markers. We can effectively expand the detection window and improve the assay's sensitivity through experimental optimization.

Experiments
  • Immune cell stimulation assay
  • Mixed lymphocyte response (MLR)
  • ELISA/Luminex cytokine detection assay
  • CFSE staining flow assay
  • Intracellular factor flow assay
  • Antigen (CMV) recall stimulation assay
Antibody-drug types Blocking/neutralizing antibodies, activating antibodies, T/NK cell engager
Stimulants CD3/CD28 antibody-coupled magnetic beads (or anti-CD3 and anti-CD28 antibodies), PMA/ionomycin, LPS, TNF-a, IL-1β, IL-2, antigen, DC, etc.
Cells PBMCs, CD3+ T cells, CD4+ T cells, CD8+ T cells, NK cells, Treg cells, dendritic cells, macrophages, etc.
Indicators Cell proliferation (CellTiter-Glo, CFSE), cytokine release (single or multiple factors detected simultaneously, ELISA/Luminex), cell surface marker protein expression or immunophenotyping, etc.
Species Humans, monkeys, mice, dogs, etc.

MLR refers to the proliferation and activation of T cells in response to antigen-presenting cell stimulation. We can evaluate histocompatibility antigen differences and the ability to respond to allogeneic cells based on the intensity of the lymphocyte response.

The general steps of MLR

The general steps of MLR - Alfa Cytology

Technology advantages

  • We have successful experience in immune cell function assays for different targets and can isolate a variety of primary cells to meet the needs of in vitro evaluation of different types of antibody drugs.
  • We have negative antibody references and positive antibody references available to our customers.
  • We provide primary cells of traceable origin to meet your filing requirements.
  • We have multiply validated and audit-trackable enzyme markers of various types.
  • We can customize our services to meet your needs and guarantee delivery lead times.

Alfa Cytology can provide a wide range of primary immune cell stimulation assays and MLR assays with a variety of assay types that can be customized to meet your needs. Please contact our staff to learn how we can serve you.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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