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Excessive inhibition or activation of immune cells is one of the reasons for the occurrence and development of brain tumors and some autoimmune diseases. Primary immune cell stimulation assay is an in vitro experimental method to screen out functionally active antibody molecules by evaluating antibodies' activating or inhibiting effect on immune cells isolated in vivo. The primary immune cell assay provides a more realistic response to the activity of antibody drugs in vivo. It helps researchers to more accurately screen for highly active antibody drug molecules during the R&D phase.
Alfa Cytology can provide primary immune cells from a wide range of stimuli and different species to help our clients assess the in vitro activity of antibodies by detecting immune cell proliferation, single or multiple cytokine releases, or the expression of immune cell surface markers. We can effectively expand the detection window and improve the assay's sensitivity through experimental optimization.
Experiments |
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Antibody-drug types | Blocking/neutralizing antibodies, activating antibodies, T/NK cell engager |
Stimulants | CD3/CD28 antibody-coupled magnetic beads (or anti-CD3 and anti-CD28 antibodies), PMA/ionomycin, LPS, TNF-a, IL-1β, IL-2, antigen, DC, etc. |
Cells | PBMCs, CD3+ T cells, CD4+ T cells, CD8+ T cells, NK cells, Treg cells, dendritic cells, macrophages, etc. |
Indicators | Cell proliferation (CellTiter-Glo, CFSE), cytokine release (single or multiple factors detected simultaneously, ELISA/Luminex), cell surface marker protein expression or immunophenotyping, etc. |
Species | Humans, monkeys, mice, dogs, etc. |
MLR refers to the proliferation and activation of T cells in response to antigen-presenting cell stimulation. We can evaluate histocompatibility antigen differences and the ability to respond to allogeneic cells based on the intensity of the lymphocyte response.
Alfa Cytology can provide a wide range of primary immune cell stimulation assays and MLR assays with a variety of assay types that can be customized to meet your needs. Please contact our staff to learn how we can serve you.