Growth Factor Analysis Services for Brain Tumors

The growth factor is a peptide with multiple biological functions. Growth factors not only bind specifically to receptors on cell membranes but also have a high affinity for the bound receptors. The uncontrolled expression of growth factors and growth factor receptors plays an important role in promoting brain tumorigenesis and development. For example, overexpression of colony-stimulating factor (CSF) and epidermal growth factor (EGF) can lead to uncontrolled proliferation, malignant transformation of certain cells, and eventual transformation into tumor cells. Meanwhile, brain tumor cells can spontaneously secrete immunosuppressive cytokines, thus the immune escape phenomenon of the tumor occurs.

We provide growth factor analysis services for brain tumors

Alfa Cytology combines enzyme-linked immunosorbent assay (ELISA), microsphere technology, and flow cytometry to provide a high throughput assay for multiple growth factors in a small number of samples simultaneously. Compared to traditional ELISA techniques, which can only detect one indicator, microsphere-based multiplex assays can detect multiple indicators from a single specimen at the same time. It saves samples and improves the efficiency of your research.

Growth factors we can test

• EGF
• Platelet-derived growth factor (PDGF)
• Osteosarcoma-derived growth factor (ODGF)
• Transforming growth factor (TGF-oL, TGFl3)
• Fibroblast growth factor (FGF)
• Insulin-like growth factor (IGF)
• Nerve growth factor (NGF)
• Interleukin-like growth factors (IL-l, IL-ll, IL-lll)
• Erythrocyte growth factor (EPO)
• CSF

General experimental flow (based on microsphere technology)

Applications

• The measurement of growth factor levels can help determine the immune function of the body, assist in the diagnosis of brain tumors, and monitor the disease process, treatment and prognosis.
• In basic research such as immunology and molecular biology, the detection of growth factor levels helps to find growth factor markers of brain tumors and further elucidate their developmental mechanisms.

Our technical advantages

• More indicators. Simultaneous testing of up to 24 indicators from a single specimen.
• Less specimen volume is required. Only 15 μl of specimens can meet the requirements of testing multiple indicators.
• Wide range of specimens. Suitable for serum, plasma, cell culture supernatant, and other body fluids.
• Free combination of assays. Allows you to freely combine assays according to your project needs.
• Same sensitivity, and a wider range. We have extended the range of tests to ensure maximum specimen applicability.

Alfa Cytology can both measure growth factor concentrations in peripheral blood and amplify the stimulatory effect of brain tumor drugs on immune cells by stimulating cells to secrete growth factors through in vitro culture, thereby predicting the potential risk that a drug may cause growth factor release syndrome. This can help you determine the minimum expected biological effect level and select a reasonable safe starting dose. Please feel free to contact us to discuss project details.