Proteomics Analysis for Brain Tumors

Cellular protein expression profiles within tissues are key to understanding disease pathology, and their spatial organization determines cellular function. The malignant brain tumor is a complex disease with multiple genes involved, and its gene expression analysis involves transcriptomic and proteomic approaches. To precisely define molecular phenotypes in the spatial context of tissues, unbiased, quantitative techniques capable of mapping the expression of hundreds to thousands of proteins within tissue structures are required.

We offer proteomic analysis services for brain tumors

To analyze the pathogenesis of brain tumors from a holistic perspective and to find specific markers for diagnosis and prognosis as well as targets for drug therapy, Alfa Cytology has applied bi-directional gel electrophoresis (2D-PAGE), high-performance liquid chromatography (HPLC), laser capture microdissection (LCM), iTRAQ markers and mass spectrometry technology. These include matrix-assisted laser resolution-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), electrospray ionization tandem mass spectrometry (ESI-MS/MS) and surface-enhanced laser resolution-ionization time-of-flight mass spectrometry (SELDI -TOF-MS ) techniques. This allows us to compare brain tumor stem cells (BTSC) with neural stem cells to find BTSC-specific markers, explore the mechanisms involved in BTSC differentiation, and provide new ideas for the origin, diagnosis, and treatment of brain tumors.

Spatial proteomics service for brain tumors

We can systematically measure the brain tumor proteome at spatial resolution using LCM technology and mass spectrometry, covering over 5,000 proteins. We offer a spatial proteomic analysis approach that addresses the need for highly multiplexed, quantitative, spatially resolved measurements of proteins within tissues. The advantage of using our solution is that no prior knowledge of tissue structure, characteristics, or pathology is required.

Protein microarray service for brain tumors

Protein microarray technology has unparalleled advantages over traditional methods such as high throughput screening, high sensitivity detection, fully automated operation, high miniaturization, and fast and efficiency. By using this technology, we can simultaneously detect and analyze multiple proteins related to brain tumors, and thus efficiently obtain protein information in brain tumor patients on a large scale. By combining MS technology and protein microarrays, we can rapidly generate and analyze protein profiles or enzymatic profiles using a small number of brain tumor cells.

We first dot-blot the protein molecules on the media according to the designed array and react to the sample proteins labeled with fluorescence with the microarray. The fluorescence signal is scanned and analyzed by a laser confocal microscope and CCD camera, and the final results are obtained.

Research goals we can help you achieve

• Search for differentially expressed proteins to discover brain tumor-related proteins.
• Search for brain tumor-related marker molecules for diagnosis.
• Search for brain tumor-related protein targets for drug design.
• To study the pathogenesis of brain tumors.

Advantages of our technology

• One experiment can output thousands of levels of data and provide qualitative, quantitative, and post-translational modification information simultaneously. This allows us to elucidate the function and mechanism of action of brain tumor-associated proteins from a more comprehensive, holistic, and network perspective.
• We can better distinguish the isoforms and modification changes of target proteins from protein-based chemical composition resolution of proteins.
• The lack of antibody limitations gives us a unique advantage in the discovery and study of novel functions of brain tumor-associated proteins.
• By combining other molecular, cellular and pathological techniques, our proteomic blood analysis of brain tumors provides an effective basis for elucidating the complex biological processes of brain tumors.

Alfa Cytology always tries to apply several technologies to provide brain tumor spatial proteomics services and protein microarray services to help customers get groundbreaking information about brain tumors. Please feel free to contact our staff for customized analysis solutions.