Single Cell Sequencing for Brain Tumors

Single-cell sequencing is cell-by-cell RNA transcription that can provide more similar insights into brain tumor biology. It can reveal mutations and structural variants that occur in the genomes of brain tumor cells, which often have extremely high mutation rates. Single-cell sequencing technology helps researchers dissect the heterogeneity of cells. With this information, researchers can characterize the clonal structure of brain tumor cells and track the progression and extent of disease spread.

Our single-cell sequencing services for brain tumors

Many brain diseases in the field of brain research lack single-cell studies, and the interactions between various types of cells in developing the disease need to be urgently explored. Alfa Cytology offers single-cell sequencing technology that can provide insight into the pathogenesis of brain tumors in terms of cell types and multi-omics, and offer new ideas for tumor prevention and treatment.

Basic services flow

Single-cell suspension preparation

• Tissue cell lysis

• Enrichment

• Quality control

Methods: cell counter, flow cytometry.

Evaluation metrics: cell size, viability, presence of aggregates, appropriate concentration.

Single-cell isolation

Amplification methods for single-cell analysis

• Transcriptome. Full-length RNA-seq, mRNA end-tag amplification, targeting panel, IR-seq.
• Genome. MDA, MALBAC, DOP-PCR, targeting panel.
• Epigenome. ATAC-seq, HiC.
• Protein detection. AbSeq, CITE-seq.

Data analysis

Application areas

• Identify glial cell diversity, including oligodendrocyte lineage, functional status of microglia, and neuronal diversity.
• Comparing human sequencing data with that of mice or non-human vertebrates also reveals markers of those conserved or specific cell types, including the proportion of cells in tissues and species specificity in gene expression of homologous cells.
• The construction of brain atlases, the study of cell types such as hippocampus, cortex, and organoid, and the study of the risk of occurrence and treatment of diseases such as tumors, aging, and cognitive and mental disorders.

Alfa Cytology offers single-cell sequencing technology that allows you to take a new perspective on brain tumor and neuroscience problems and can help you discover genomic variants in brain tumor cells. It will help you to deepen your understanding of the cellular state, the nature of transcription and regulation of gene expression, and even the pathological process of brain tumors. Please contact our staff to learn how we can do this.