What We Offer
Developing a sensitive, label-free biosensor requires coordinated expertise in sensor architecture design, surface chemistry, antibody immobilization, and electrochemical characterization. Our Biosensor Development and Validation Service provides diagnostic companies and academic research labs with end-to-end support for creating new electrochemical immunosensor platforms—including those targeting cancer biomarkers such as prostate-specific antigen (PSA).
Built on proven CRO methodologies and guided by industry-standard validation frameworks (e.g., high-content 96-well plate assays; see literature), the program covers every stage from sensor design through analytical performance verification. We work collaboratively with your team to translate a detection concept into a functional, reproducible prototype, with detailed documentation suitable for downstream assay transfer.
Whether you are optimizing a novel polymeric network (e.g., carbazole-pyranine derivatives) or adapting an existing sensor to a new analyte, our scientists bring hands-on experience in electrochemical testing, surface functionalization, and data-driven optimization to reduce development timelines. Validation includes assessment of sensitivity, linear range, limit of detection, selectivity, and batch reproducibility, ensuring the platform is ready for further preclinical or clinical application.
Service Scope
Sensor Design & Fabrication
We assist in the selection of electrode materials, design of the recognition layer, and fabrication of prototype sensors. Support includes integration of electroactive polymeric networks and optimization of surface area for enhanced signal transduction.
Antibody Immobilization & Surface Chemistry
Development of stable, oriented immobilization protocols for capture antibodies or aptamers on the sensor surface. Validation of binding activity and reproducibility across multiple sensors.
Electrochemical Testing & Validation
Quantitative characterization using cyclic voltammetry, electrochemical impedance spectroscopy, and amperometric detection. Performance verification against target biomarkers (e.g., PSA) with assessment of sensitivity, linear range, and selectivity.
Our Collaborative Process
We discuss your target biomarker, sensor architecture preferences (e.g., electrode format, polymeric network), performance targets (sensitivity, dynamic range, selectivity), and intended application. Together we define the scope of development and validation studies, including the number of prototype iterations, analytical endpoints, and a detailed project timeline.
Based on the approved design, our team fabricates prototype sensors using techniques such as electrochemical polymerization of carbazole-pyranine networks, nanomaterial deposition, or photolithography. Each fabrication run is documented for quality control, and preliminary electrochemical testing confirms proper function before proceeding.
We immobilize capture molecules (antibodies or aptamers) using optimized chemistry (e.g., EDC/NHS coupling, avidin-biotin) to ensure uniform surface coverage and retained bioactivity. Process parameters—including concentration, incubation time, and blocking conditions—are optimized and documented for reproducibility.
The sensors are characterized by cyclic voltammetry and impedance spectroscopy to confirm successful fabrication and functionalization. Baseline signals, electrode-to-electrode variability, and batch-to-batch consistency are recorded. This step ensures that each sensor meets predefined acceptance criteria before proceeding to analyte testing.
We perform dose‑response measurements with your target biomarker to establish sensitivity, linear dynamic range, limit of detection, and selectivity against potential interferents. A high‑content 96‑well plate assay format may be used for efficient validation, enabling multiple conditions and replicates to be assessed simultaneously. Data analysis includes curve fitting, statistical evaluation, and determination of performance parameters.
You receive a comprehensive validation report including all raw data, calibration curves, statistical analysis, and step‑by‑step protocols for sensor fabrication and testing. The prototype sensors (quantity scoped per project) are shipped with the report. All documentation is structured for easy transfer to your quality management system or regulatory submission package.
Deliverables
| Item | Description |
|---|---|
| Sensor Prototypes | Fabricated and functionalized sensor units (quantity scoped per project) suitable for immediate testing in your lab. |
| Electrochemical Data Package | Full cyclic voltammetry and impedance spectra, including raw files with analysis parameters. |
| Validation Report | Detailed report covering calibration curves, LOD, linear range, selectivity, and batch reproducibility. |
| Protocols & SOPs | Written protocols for sensor fabrication, immobilization, and measurement procedures for in‑house replication. |
Why Choose This Service
Comprehensive SELEX-to-Biosensor Integration
Our CRO approach combines aptamer discovery via SELEX with downstream biosensor development, enabling streamlined transition from capture molecule identification to sensor integration.
High-Content Validation Readiness
We adopt validated 96‑well plate high‑content assays to accelerate performance verification and generate statistically robust data across multiple conditions.
Customizable Detection Targets
Service is not limited to PSA; we can adapt the platform to other protein, nucleic acid, or small‑molecule biomarkers based on your project requirements.
End‑to‑End Documentation
Every step from design to validation is documented with standard operating procedures, ensuring traceability and smooth transfer to your own quality system.
Related Research
Latest Research
A High-Content Assay for Biosensor Validation and for Examining Stimuli that Affect Biosensor Activity
View on PubMedReady to advance your biosensor program?
Contact us to discuss your target analyte, sensor design, and validation needs. Our scientists will work with you to define a project scope that meets your development milestones.