Exosome Isolation and Characterization Services

Why Exosome Isolation and Characterization Matter

Exosomes are 30–150 nm extracellular vesicles that mediate intercellular communication by transferring proteins, lipids, and nucleic acids. In oncology, exosomes from drug-resistant tumor cells can shuttle microRNAs (e.g., miR‑941) that activate survival pathways such as the Keap1/Nrf2 axis, contributing to therapy failure. Isolating pure exosome fractions is essential to dissect these mechanisms, validate biomarker candidates, and develop exosome-based therapeutics.

Our exosome isolation and characterization service provides researchers with high-quality, size‑ and marker‑defined exosome preparations from a variety of starting materials, including serum, plasma, urine, and conditioned cell culture media. We employ validated isolation methods—ultracentrifugation, size exclusion chromatography, precipitation, or affinity capture—selected and optimized for your sample type and downstream application. Each preparation is rigorously characterized for particle size, concentration, morphology, and enrichment of exosome markers (CD9, CD63, CD81) using orthogonal techniques.

Deliverables include a detailed characterization report with quantitative data, enabling you to confidently proceed to functional assays, cargo profiling, or biomarker analysis. Whether your goal is to study how exosomes transfer drug resistance, identify circulating biomarkers, or explore the role of extracellular vesicles in disease, our service provides the reliable, reproducible exosome material you need.

Core Service Components

Flexible Isolation Methods

Choose from ultracentrifugation, size exclusion chromatography, precipitation, or immunoaffinity capture. Each method is optimized for your sample volume and purity requirements, with protocols tailored to biofluid or cell culture media.

Comprehensive Characterization

Particle size distribution and concentration measured by nanoparticle tracking analysis (NTA), morphology confirmed by transmission electron microscopy (TEM), and marker enrichment validated by Western blot or flow cytometry for CD9, CD63, and CD81.

Downstream-Readied Deliverables

Purified exosome fractions supplied with a full characterization report. Optional extraction of RNA or protein from the isolated exosomes can be included to accelerate your cargo analysis or functional studies.

Engagement Workflow

Sample Preparation and Clarification Your sample (serum, plasma, urine, or conditioned medium) is centrifuged at low speed to remove cells and large debris. A subsequent high-speed spin clears larger microvesicles, leaving a clarified supernatant enriched for small extracellular vesicles.

Exosome Isolation Isolation is performed using a validated method selected for your project. Ultracentrifugation pellets exosomes at high g‑forces; size exclusion chromatography (SEC) separates them by size; precipitation reagents concentrate vesicles; or affinity capture enriches via surface markers.

Quantification, Sizing and Morphology Nanoparticle tracking analysis (NTA) determines particle concentration and size profile. Transmission electron microscopy (TEM) confirms vesicular morphology and integrity. Dynamic light scattering (DLS) may be added for orthogonal size measurement.

Marker Validation and Reporting Enrichment of canonical exosome markers (CD9, CD63, CD81) is assessed by Western blot or flow cytometry. A final report summarizes all characterization data, including yield, purity, and marker positivity rates, to confirm the exosome preparation meets your specifications.

Deliverables and Service Scope

Parameter	Typical Project Scope
Starting material	Serum, plasma, urine, cell culture conditioned medium (volume scoped per project)
Isolation method	Ultracentrifugation, SEC, precipitation, or affinity capture (selected upon consultation)
Particle concentration and size	NTA report with particle/mL and mean/mode size distribution
Morphological assessment	TEM images at specified magnifications (number of fields scoped per project)
Marker validation	Western blot for CD9, CD63, CD81; optional flow cytometry phenotyping

Why Choose Our Exosome Services

Method Flexibility

We do not lock you into a single approach. Each project begins with a consultation to select the isolation and characterization pipeline best suited to your sample type, purity needs, and downstream assay—whether that is ultracentrifugation, SEC, precipitation, or immunoaffinity capture.

Orthogonal Characterization

True exosome identity is not defined by size alone. We combine NTA, TEM, and marker analysis (CD9/CD63/CD81) to confirm both physical and molecular criteria, ensuring you receive exosome fractions that meet MISEV guidelines for research.

Project‑Specific Optimization

Sample composition varies widely. Our protocols are fine‑tuned for each starting material—for example, adapting centrifugation speeds for viscous plasma or adjusting SEC column load for concentrated conditioned media—to maximize yield and purity.

Comprehensive Reporting

Every deliverable includes a detailed characterization report with raw data and images. This enables you to confidently compare quality across batches, support publication requirements, and make informed decisions for subsequent functional experiments.

Read the Study

miR-941 in extracellular vesicles confers anlotinib resistance via Keap1/Nrf2 axis and represents a therapeutic target in non-small cell lung cancer

View article

Start Your Exosome Isolation Project

Ready to obtain pure, well‑characterized exosomes for your biomarker, drug resistance, or mechanistic studies? Contact our team to discuss your sample type, desired downstream application, and project goals. We will design a custom isolation and characterization plan that meets your research requirements.

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