What TCR Repertoire Sequencing Delivers for Vaccine Research
TCR repertoire sequencing is a targeted next-generation sequencing service that profiles rearranged T-cell receptor chains, most commonly by resolving CDR3 regions to identify clonotypes and measure repertoire diversity. Projects can cover TCR alpha, beta, gamma, and delta chains depending on the sample type and study goals. For cancer vaccine research, the resulting clonotype tables and diversity metrics support tracking of T-cell expansion, repertoire shifts, and treatment- or vaccine-associated immune responses. We accept T-cell-containing samples such as blood, PBMC, and tumor tissue samples from human or preclinical studies, and we review sample suitability before library preparation so that sequencing depth and analysis scope match the question being asked.
Pairing repertoire data with antigen-specific T cell response assays can connect clonal changes with measured antigen-responsive activity.
What Our TCR Repertoire Sequencing Service Includes
Chain and Sample Flexibility
TCR alpha, beta, gamma, and delta chain profiling is available depending on sample type and project goals, with input from blood, PBMC, or tumor tissue reviewed for suitability before library construction.
CDR3-Targeted Library Workflow
Nucleic acid is extracted from T-cell-containing samples and prepared into TCR-targeted libraries, typically focused on CDR3 regions, then sequenced on next-generation sequencing platforms.
Clonotype and Diversity Analysis
Bioinformatic processing calls clonotypes and reports repertoire diversity and expansion metrics, supporting comparison of vaccine- or treatment-associated immune responses across timepoints or groups.
QC Review
Sequencing depth, clonality, and replicate consistency are reviewed as part of standard QC, with findings summarized alongside the analysis so results can be interpreted in context.
How a TCR Repertoire Sequencing Engagement Works
What You Receive
| Deliverable | Description |
|---|---|
| Raw sequencing data | FASTQ files for each library, with read-level quality information for downstream review or reanalysis. |
| Clonotype tables | Tabulated TCR clonotype calls, typically CDR3-focused, with chain assignment and read support per clonotype. |
| Repertoire diversity metrics | Summary diversity and clonality measures computed from the clonotype tables, scoped to the chains and samples in the project. |
| QC summary | Sequencing depth, clonality, and replicate consistency review, reported alongside the dataset so results can be interpreted in context. |
| Summary report | A written report covering sample handling, workflow, QC outcomes, and the delivered data files, prepared for cancer vaccine research teams. |
Why Teams Choose Our TCR Repertoire Sequencing Service
Chain coverage matched to your question
Projects can target TCR alpha, beta, gamma, and delta chains depending on sample type and study goals. Most cancer vaccine work centers on beta chain CDR3 profiling, while gamma-delta options are available when the research question calls for them.
Sample types relevant to vaccine studies
We accept T cells, PBMC, blood, and tumor tissue samples from human or preclinical sources for suitability review. Input amount and sample quality are matched to the library workflow before sequencing begins.
Analysis oriented to immune response tracking
Clonotype tables and diversity metrics are structured to support tracking of T-cell expansion, repertoire shifts, and treatment- or vaccine-associated immune responses. Interpretation of biological meaning remains with your research team, and results are project-dependent.
Further Reading
Research reference
1. RNA neoantigen vaccines prime long-lived CD8+ T cells in pancreatic cancer
Nature. 2025. DOI: 10.1038/s41586-024-08508-4.
View articlePlan a TCR Repertoire Sequencing Project for Cancer Vaccine Research
Share your sample types, species, chain preference, and the immune questions your vaccine study needs to address. Our team will review sample suitability, propose a CDR3-targeted sequencing and analysis scope, and outline the deliverables and QC checkpoints for your project.
Human sample projects can include PBMC isolation and immune cell preparation to coordinate immune-cell preparation with sequencing.