General Information
| Alternative Names | Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6 |
| Target Name | Alpha smooth muscle actin |
| Description | Recombinant rabbit monoclonal antibody recognizes alpha-smooth muscle actin |
| Species Reactivity | Hu, Ms, Rat, AGMK, Bov, Dog, Pig, Zfsh |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SQab18108 |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Synthetic peptide corresponding to residues on the N-terminus of human alpha smooth muscle actin. |
| Conjugate | Un-conjugated |
| Application | FACS, ICC/IF, IHC-P, WB |
| Package | 100 μL |
| Form | Liquid |
| Purification | Purification with protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for WB | 1:1000 - 1:2000 |
| Recommended Dilution for IHC-P | 1:2000 - 1:4000 |
| Recommended Dilution for IF | 1:80 - 1:200 |
| Recommended Dilution for ICC | 1:80 - 1:200 |
| Recommended Dilution for FACS | 1:20 - 1:100 |
| Application Notes | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | ACTA2 |
| Gene Full Name | Actin, alpha 2, smooth muscle, aorta |
| Background | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. |
| Biological Functions | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| Research Field | Cell biology and cellular response antibody; Controls and markers antibody; Signaling transduction antibody; Cancer-associated fibroblast antibody; Smooth muscle marker antibody; CAF marker antibody; Mural cell marker antibody; Myofibroblast marker antibody; Angiogenesis study antibody; Microvascular density study antibody |
| Predict Molecular Weight | 42 kDa |
| Post-Translational Modification | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt] |
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