Genetically Modified Tumor Models for TME Research
Relying on the tumor microenvironment center technology platform, Alfa Oncology provides customized services for genetically modified tumor animal models for TME research, helping researchers successfully achieve their research goals.
Different tumor animal models differ in their oncological traits, and we should choose the most suitable animal model with the appropriate experimental protocol according to the set experimental goals and specific objectives when doing research in order to obtain scientific conclusions and ideal experimental results. Gene-modified tumor models are models that use techniques such as transgenesis, gene targeting and conditional gene targeting to knock out or insert specific genes, thereby inducing tumors in animals. They are generally used mainly for the study of precancerous lesions and the process of tumor development and mechanism of action, and can also be applied to anti-tumor drug screening. It is particularly advantageous in the study of tumors and microenvironment.
Genetically engineered models are animals with genetic alterations in their DNA and are important and powerful tools for studying the causes of tumors and microenvironmental cures and for developing therapeutic interventions. An ideal model based on a specific genetic background is always the cornerstone of your research.
At Alfa Oncology, we have established a mature tumor microenvironment center technology platform, a group of experienced tumor model customization experts, and mature gene editing technologies (such as CRISPR/Cas9, TALEN, ZFN, ES) to build various types of gene editing mouse models such as transgenic, knockout, and knock-in. At present, we have completed tens of thousands of cases of gene editing mouse models, providing customers with stock model products, model customization, joint R&D and other services to provide you with the best genetic engineering models to meet your exact expectations.
The customization services we offer for genetically modified tumor models include:
Knockouts are manipulations of DNA that result in the loss of function or complete loss of the corresponding protein. Knockout models are "loss-of-function" mutants. Generally, they can be divided into three types: conventional knockout models, conditional knockout models and KO first (condition-ready) models.
For example: P53 KO, P53 CKO, Pten KO, Pten CKO, Smad4 CKO, Rb1 CKO, Brca1 CKO, etc. In addition to the above tumor mouse models, Alfa Oncology will continue to release more tumor-related genetically engineered mouse models, so please stay tuned.
Random transgenic models can be obtained by transposon-mediated insertion, DNA microinjection and lentivirus-mediated transfection methods. Knock-in models have genetic elements inserted into specific motifs in the genome. Exogenous genes can be targeted into embryonic or ES cells in the form of point mutations and cassette insertions (e.g., reporter genes), resulting in "gain-of-function" mutants.
For example: Myc conditional overexpression, Apc-(L850X), Kras(G12D), KPC model, etc.
Gene knockdown is a technique used to reduce the expression of one or more genes in an organism, resulting in temporary functional neutralization. This can be achieved through gene modification or the use of short RNA oligonucleotides (e.g. siRNA) that are complementary to the target mRNA to be silenced.
The main technologies used in our genetically modified tumor model customization service are:
The ES cell-mediated targeting technique for obtaining gene editing mouse models is more suitable than the CRISPR/Cas9 technique for making mouse models with complex editing strategies or large insertion fragments.
CRISPR/Cas9 technology is the most commonly used gene editing technology for the preparation of CKO/KO/KI/PM mice with specific gene modifications.
Genetically engineered mouse models are obtained by random integration of DNA into the mouse genome and are generally used to obtain the preparation of overexpressing or conditionally overexpressing mice. With the increasing efficiency of K, most investigators have switched to the use of H11 or Rosa26 locus targeted knock-in for the production of overexpression mouse models.
Note: Our services list is constantly being updated and improved, please contact us by email for more up-to-date and relevant information.
- This class of models provides excellent research tools to study the role of the tumor microenvironment in tumor development and treatment.
- This class of models provides excellent material for the study of the interaction between environmental factors and genetic background.
- This class of models has great advantages for studying the mechanism of tumorigenesis, tumor immune evasion, and transgenic models.
- This type of model requires long time and high cost to establish.
Alfa Oncology is dedicated to supporting scientists in making groundbreaking scientific discoveries and developing new applications to accelerate new drug discovery and scientific diagnosis and treatment. We have established the tumor microenvironment center technology platform with high performance scientific instruments and high value solutions to ensure high quality services that enable scientists to explore the mysteries of life at the tumor microenvironment level. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. If you have any questions, please feel free to contact us.