Imaging Services for Angiogenesis in TME
The creation of new blood vessels from existing ones, known as angiogenesis, is a critical process in the tumor microenvironment (TME). It supports the rapid growth and metastasis of cancer cells by providing oxygen, nutrients, and facilitating migration. Recognizing the importance of angiogenesis, Alfa Cytology provides customized imaging services and products to study this crucial process in the TME.
The Study of Angiogenesis in TME
The intricate vascular network existing within the TME frequently exhibits abnormal vessel structure, increased permeability, and irregular blood flow. Delving deep into the complicated processes of angiogenesis within the TME becomes an indispensable endeavor, as it is crucial for comprehending tumor biology, evaluating treatment responses and developing innovative therapeutic strategies. An array of cutting-edge imaging methodologies, including intravital microscopy, multiphoton microscopy, dynamic contrast-enhanced MRI (DCE-MRI), contrast-enhanced ultrasound (CEUS), and molecular imaging modalities such as positron emission tomography (PET), single-photon emission computed tomography (SPECT) bestow upon us the opportunity to visualize multitudinous facets of angiogenesis within the TME with high-resolution resolution. By combining molecular targeting, functional assessments, and advanced image analysis, angiogenesis imaging services provide valuable data on the spatial and temporal characteristics of angiogenesis, molecular targets, treatment efficacy, and anti-angiogenic therapy development.
Fig. 1 Angiogenesis Targets in the Tumour Microenvironment. (Florea A, et al., 2021)
Angiogenesis Imaging Services
Alfa Cytology offers comprehensive imaging services to visualize, quantify, and monitor angiogenesis in the TME. Our services support researchers in studying and understanding angiogenesis in the TME, aiding in the identification of therapeutic targets for effective anti-angiogenic therapies. Our services include, but are not limited to:
| Services | Techniques | Description |
|---|---|---|
| Microvessel density (MVD) analysis | Computerized tomography (CT) Contrast-enhanced ultrasound (CEUS) Magnetic resonance imaging (MRI) Optical coherence tomography (OCT) Positron emission tomography (PET) Flow cytometry Intravital microscopy |
Our imaging techniques provide quantitative information about the density of microvessels within the TME, which serves as an indicator of angiogenic activity. |
| Vascular permeability assessment | Dynamic contrast-enhanced MRI (DCE-MRI) Fluorescence imaging Near-infrared fluorescence (NIRF) imaging |
By measuring vascular leakage and permeability, we gain important information about the extent of angiogenesis and the formation of abnormal blood vessels within the TME. |
| Blood flow analysis | Doppler ultrasound Dynamic contrast-enhanced MRI (DCE-MRI) Optical imaging techniques CT |
By analyzing the velocity of blood flow and evaluating the perfusion of blood vessels, we can offer a comprehensive evaluation of the dynamics of angiogenic sprouting, the maturation of vessels, and the effectiveness of delivery to the tumor. |
| Vessel morphology and architecture evaluation | 3D reconstruction High-resolution microscopy Micro-computed tomography (Micro-CT) CT MRI |
We assess parameters such as vessel diameter, length, branching, and curvature, providing valuable insights into the process of angiogenesis within the TME. |
| Molecular imaging of angiogenic markers | Fluorescence imaging Optical imaging techniques Single photon emission computed tomography (SPECT) MRI PET |
We possess the capability to visualize and quantify the expression and activity of specific molecular targets involved in angiogenesiswithin the TME, including vascular endothelial growth factor (VEGF), αvβ3 integrin, Endoglin (CD105), hypoxia-inducing factor (HIF), and matrix metalloproteinases (MMPs). |
The Advantages of Our Angiogenesis Imaging Services
- We offer a range of services from in vitro studies employing cancer cell lines to in vivo characterization of angiogenic response using experimental models.
- Our high-resolution imaging systems, such as confocal and multiphoton microscopy, allow us to visualize angiogenic sprouting, branching, and vessel maturation in three-dimensional resolution.
- Our versatile microfluidic platforms allow us to mimic the TME accurately, thereby facilitating studies on microenvironmental influences on tumor angiogenesis.
- We provide customized imaging solutions to align with your project objectives and research requirements.
At Alfa Cytology, we recognize the significance of unraveling the complexities of angiogenesis, a fundamental process that contributes to tumor growth and metastasis. Our top-tier imaging services provide a window into angiogenesis within the TME, equipping you with high-resolution visualizations and precise quantification. Your research goals are our priority, and we are committed to providing customized solutions to accelerate your discovery process. Take the next step in your angiogenesis research. Contact us today to learn more about our imaging services and how they can propel your research forward.
Reference
- Florea A, et al. Molecular Imaging of Angiogenesis in Oncology: Current Preclinical and Clinical Status. International journal of molecular sciences vol, 2021, 22(11): 5544.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.