Analysis of Neutrophil Functions in TME

Among the immune cell populations present in the tumor microenvironment (TME), neutrophils emerge as key players in mediating inflammation and influencing tumor behavior. Alfa Cytology provides researchers with an extensive range of assays focused on neutrophils, enabling the deciphering of the complex roles played by these cells within the TME.

The Role of Neutrophils in TME

Neutrophils display remarkable adaptability in different inflammatory contexts, including cancer. They possess the capacity to secrete proteases, chemokines and cytokines, thereby contributing to cancer progression. Neutrophils also release reactive nitrogen species (RNS) and reactive oxygen species (ROS), further amplifying their impact on the TME. Additionally, neutrophils can facilitate cancer progression through the formation of neutrophil extracellular traps (NETs). NETs are web-like structures composed of DNA, histones, and antimicrobial proteins released by neutrophils. Emerging research indicates that NETs can promote tumor cell extravasation, metastasis, and immune evasion. Through in-depth analysis of the diverse functions of neutrophils, researchers can acquire significant knowledge about the mechanisms that drive cancer advancement.

Neutrophil-released factors in the tumor microenvironmentFig. 1 Neutrophil-released factors in the tumor microenvironment. (Wu L, et al., 2020)

Analysis of Neutrophil Functions in TME

At Alfa Cytology, we offer a range of scientifically validated neutrophil assays tailored to investigate their functions and interactions in the TME. Our services include, but are not limited to:

  • Neutrophil Phenotypic Characterization
    We employ flow cytometry techniques to identify and quantify distinct subsets of tumor-associated neutrophils (TANs) based on specific cell surface markers and functional markers. By examining their expression of immune checkpoint molecules, such as PD-L1, we can gain a comprehensive understanding of the immunomodulatory capabilities of TANs and their involvement in tumor immune evasion.
  •  Neutrophil Functional Assays
    To elucidate the functional implications of neutrophils in the TME, we employ a range of functional assays based on flow cytometry and real-time imaging to assess their proinflammatory or suppressive properties. These assays include measuring cytokine secretion profiles, phagocytic activity, reactive oxygen species production, and neutrophil extracellular trap formation. By deciphering the functional behavior of neutrophils in the TME, we can uncover their contribution to tumor progression and immune modulation.
  • NET Formation Assays
    NETs have been implicated in tumor progression and immune regulation. We employe fluorescence microscopy and DNA staining techniques to visualize and quantify NET formation within the TME, facilitating in-depth mechanistic studies. By studying the formation and impact of NETs in the TME, you can gain a deeper understanding of their role in cancer development and explore potential therapeutic targets.
  • Analysis of Neutrophil Interactions with Tumor or Immune Cells
    Neutrophils can directly interact with tumor cells, influencing their behavior and promoting or inhibiting tumor growth. And investigating the interactions between neutrophils and other immune cells, such as T cells, is crucial for unraveling the complex dynamics within the TME. At Alfa Cytology, we utilize co-culture assays and advanced imaging techniques, such as confocal microscopy and live-cell imaging, to visualize and analyze the dynamic interactions between neutrophils and other cells. By studying these interactions in real-time, we gain insights into the mechanisms underlying neutrophil-mediated effects on tumor cells and their immune modulation potential.

The Advantages of Our Neutrophil Function Analysis

  • At Alfa Cytology, we offer a comprehensive panel of assays to evaluate neutrophil function and phenotype. From phagocytosis and chemotaxis to reactive oxygen species (ROS) production and cytokine secretion, our assays provide in-depth insights into the diverse functions of neutrophils in the TME.
  • We employ advanced flow cytometry platforms and advanced imaging techniques enable precise and accurate measurement of neutrophil parameters in the TME. By leveraging these technologies, we ensure that your research is built on a foundation of excellence and innovation.
  • Our assays enable the investigation of the complex interplay between neutrophils and tumor cells and neutrophil-mediated immune suppression mechanisms within the TME, facilitating the development of targeted immunotherapies.

Alfa Cytology offers validated research methods and assays that empower researchers to delve into the precise mechanisms and functions of neutrophils in the TME. By availing our services, you can gain valuable insights that will advance the understanding of neutrophil involvement in cancer progression. Contact us today to discuss your unique research requirements and unlock crucial discoveries.


  1. Wu L, et al. Neutrophils in the Tumor Microenvironment. Adv Exp Med Biol, 2020;1224:1-20.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.


Alfa Cytology is a service provider specializing in tumor microenvironment research.

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