Solutions for Inflammatory Response Pathways in the TME
Relying on the technology platform of the tumor microenvironment center, Alfa Oncology is committed to providing global collaborators with comprehensive solutions and services for the study of inflammatory response signaling pathways in the tumor microenvironment (TME) to help you successfully achieve your research goals.
With the increasing understanding of tumors, the focus of tumor treatment is not only on the tumor cells themselves, but also on the tumor microenvironment, which is the "growing and germinating soil" of tumor cells. Inflammation, as a key link in the formation of tumor microenvironment (TME), has been shown to play an important role in tumorigenesis and development. Tumor inflammatory signaling pathways are recently discovered to regulate organ volume and maintain the balance of cell proliferation and apoptosis, and their deregulation can lead to the occurrence and development of various tumors in humans. It has been shown that multiple inflammatory signaling pathways are cross-linked and involved in the development of certain tumors. However, the network relationships among inflammatory pathways, the role of signal transduction in promoting the progression of inflammation or repairing damaged tissues, and the processes through which their abnormal regulation affects tumors and their microenvironment are worth further investigation.
There are four phases of the inflammatory response: inflammatory inducers (infection or tissue damage), inflammatory receptors (mast cells and macrophages), inflammatory mediators (cytokines such as TNF, VEGF, IL-1, chemokines such as CXCL12, CCL27, CCl21, others), and affected tissues. There are many options for each phase, all of which involve communication of complex molecular signaling cascade response pathways. An enhanced understanding of the signaling pathways of the tumor inflammatory response will allow us to better develop new anti-tumor tools to combat tumors. Alfa Oncology has established an innovative technology platform for the tumor microenvironment center and is developing multiple technologies to provide high quality solutions and services to our global collaborators. By delving into the signaling pathways of the inflammatory response in the tumor microenvironment (TME), we expect to identify new targets and develop new approaches for combination therapies.
Multi-omics integration analysis of tumor inflammation signaling pathways. Statistical analysis such as normalization, comparative analysis and correlation analysis are performed on batch data from different biomolecular levels such as genome, transcriptome, proteome, metabolome and lipidome to establish data relationships between different levels of molecules. We also combine GO functional analysis, metabolic pathway enrichment, molecular interactions and other biofunctional analyses to systematically and comprehensively analyze biomolecular functions and regulatory mechanisms. The integration and analysis of histological data at different molecular levels can, on the one hand, verify each other and, on the other hand, help to complement each other, and finally achieve a comprehensive understanding of the general trends and directions of biological changes, propose models of molecular biological change mechanisms, and screen out key metabolic pathways or proteins, genes, metabolites, and lipid products for subsequent in-depth experimental analysis and application.
Alfa Oncology uses viral technology to assist in the study of tumor inflammatory signaling pathways. Lentiviral vectors are used to construct and screen stable transfected cell lines for the study of signaling pathway activity. By stably transfecting cells with reporter genes whose expression is controlled by key transcription factors of signaling pathways, such as EGFP and luciferase, the activation and inactivation status of the corresponding signaling pathways in cells can be detected visually and conveniently. It can be used for corresponding cellular receptor agonist and antagonist experiments, multiple cellular function experiments, etc.
Alfa Oncology also uses the following techniques to assist in the study of tumor inflammatory signaling pathways. Intervention techniques for intracellular signaling molecules, protein kinase phosphorylation and its activity determination, protein phosphorylation site analysis and its functional identification, cell signaling molecule specific inhibitors, fluorescent labeling of cell signaling molecules, 3D structural analysis of cell signaling molecules, protein-nucleic acid interaction study techniques, signaling molecule gene expression detection techniques, etc.
Alfa Oncology is committed to supporting scientists in making breakthrough scientific discoveries and developing new applications to accelerate new drug discovery and scientific diagnosis and treatment. Our high-performance scientific instruments and high-value solutions enable scientists to explore the mysteries of life at the tumor microenvironment level. Please tell us your project requirements, and we will provide you with a full service from solution to report. If you have any questions, please feel free to contact us.