Patient-Derived Organoids for TME Research
Relying on the tumor microenvironment center technology platform, Alfa Oncology provides customized services for patient-derived organoids for TME research, helping researchers successfully achieve their research goals.
The construction of a tumour microenvironment (TME) to study tumour heterogeneity, tumour progression and subsequent metastasis, drug response and drug resistance has been a great challenge in cancer research. There are two effective research models, patient-derived xenografts (PDX) and patients derived organoids. Patient-derived organoids are miniature 3D tumor cell models that are cultured in the laboratory from primary tumors in patients with the disease.
Patients derived organoids can encapsulate the tumor microenvironment including tumor cells and non-tumor host components, and are phenotypically and genetically similar to the tumor tissue from which they originate, highly mimicking the characteristics of the source tumor tissue, preserving tumor heterogeneity between individuals, and can be used for mechanistic studies and functional testing.
Fig. 1 Organoid culture strategies for modeling the tumor (immune) microenvironment
With the development of stem cell biology, in vitro 3D cell culture differentiation into organoids that mimic the spatial morphology of in vivo organs can also be applied to the development of new human tumor models. At Alfa Oncology, we have established a mature tumor microenvironment center technology platform with a group of experienced tumor model customization experts and proven technologies designed to provide our global customers with efficient and validated tumor models for tumor and microenvironment research and antitumor drug discovery.
Alfa Oncology offers solutions and services for the construction of patients derived organoids, covering cell lines, substrates and consumables, media systems (extracellular matrix (ECM) proteins and different growth factors), and imaging analysis platforms and reagents to help you explore the relationship between tumor development and the microenvironment.
- Patient-derived organoid
- Brain organoid
- Intestinal organoids
- Lung organoids
- Automated laboratory workflow for organoid screening
1. 2D preculture
Organoids are derived from primary cells (i.e. intestinal, lung or kidney cells) or induced pluripotent stem cells.
2. Development into 3D organoids
The medium includes extracellular matrix (ECM) proteins and different growth factors, which will vary depending on the type of tissue being cultured.
3. Organoid culture
Organoid culture is a lengthy process that may involve different steps and the use of different media. During this process, the health of the cells needs to be monitored (imaged), generally to understand developmental biology and tissue.
4. Monitoring and readings
With high-intensity imaging, it is possible to monitor and observe the growth and differentiation of organoids, 3D reconstruction of structures, complex analysis of organoid structures, cell morphology and activity, and the expression of different cellular markers.
5. Confocal imaging and 3D analysis
Confocal imaging and 3D analysis of organoids allows the observation and quantification of organoids and the cells that make up organoids. Multiple quantitative descriptions of organoids can be used to study disease phenotypes and compound effects.
Note: Our services list is constantly being updated and improved, please contact us by email for more up-to-date and relevant information.
With extensive experience in various cancer models and expertise in tumor and microenvironment, Alfa Oncology is the ideal partner to support your oncology therapeutic research. We provide customers with stock model products, model customization, and joint R&D services to provide you with the best tumor models and look forward to meeting your precise needs. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. If you have any questions, please feel free to contact us.
- Xia T, Du W, Chen X, et al. Organoid models of the tumor microenvironment and their applications[J]. Journal of Cellular and Molecular Medicine, 2021(7).