Imaging Services for Cytokines in TME
The tumor microenvironment (TME) plays a crucial role in tumor development, progression, and response to therapy. Within the TME, various cellular components and signaling molecules interact to regulate tumor growth, immune responses, and therapeutic resistance. Cytokines, a diverse group of small proteins, are key players in mediating these interactions. At Alfa Cytology, we offer advanced characterization services for cytokine imaging in the TME.
The Study of Cytokines in TME
Cytokines are critical signaling molecules that regulate immune responses, inflammation, cell proliferation, and survival. In the context of cancer, cytokines play a key role in tumor progression, immune evasion, angiogenesis, and metastasis. Cytokines can be produced by different cell types, including tumor cells, immune cells, and stromal cells. The localization of cytokines can vary within different tumor regions, such as the tumor core, invasive front, or peritumoral areas. By examining cytokine expression and spatial heterogeneity within the TME, researchers can gain insights into the complex signaling networks that influence tumor behavior and immune cell interactions. Imaging techniques play a crucial role in uncovering the diverse distribution and varying levels of cytokine expression.
Fig. 1 Role of cytokines/chemokines on tumor progression and dissemination. (Cords L, et al., 2021)
Cytokine Imaging Services
By employing cutting-edge imaging techniques, multiplexed assays, spatial analysis, and data integration, Alfa Cytology offers advanced characterization services for cytokine imaging in the TME. Our services include, but are not limited to:
| Services | Techniques | Description |
|---|---|---|
| Cytokine Expression Profiling | Immunofluorescence imaging Multiplexed Imaging In situ Hybridization (ISH) Positron emission tomography (PET) Single-photon emission computed tomography (SPECT) |
Our imaging techniques allow us to visualize, localize, and quantify cytokines, providing our clients with a comprehensive understanding of their distribution and abundance across various scales. |
| Cytokine Signaling Pathways | Immunofluorescence Imaging Förster resonance energy transfer (FRET) Fluorescence lifetime imaging microscopy (FLIM) Single-cell imaging and high-content analysis Live cell imaging |
In conjunction with relevant experimental models and genetic or pharmacological perturbations, we employ these imaging techniques to gain valuable insights into the intricate cytokine signaling pathways operating within the TME. |
| Interactions with Immune Cells | Flow Cytometry High-resolution microscopy Live Cell Imaging Co-culture Systems |
We can visualize, quantify, and characterize the behavior, localization, and functional outcomes of immune cells in the TME in response to specific cytokines. |
The Advantages of Our Cytokine Imaging Services
- We offer a comprehensive suite of services, covering both in vivo and in vitro studies, enabling precise investigations into the dynamic behavior and interactions of cytokines in the TME.
- Our state-of-the-art imaging systems, including confocal microscopy and multiphoton microscopy, provide exceptional resolution, enabling the visualization of cytokines within the complex three-dimensional architecture of the TME.
- We employ advanced algorithms and software tools to quantify and evaluate cytokine expression patterns, gradients, and associations with tumor cells and stromal elements.
With our commitment to precision, expertise, and customized solutions, Alfa Cytology is dedicated to advancing our understanding of cytokine-mediated processes in the TME and facilitating the development of novel therapeutic strategies for cancer treatment. Contact us today to learn more about our imaging services how we can support your research.
Reference
- Bhat AA, et al. Cytokine-chemokine network driven metastasis in esophageal cancer; promising avenue for targeted therapy. Mol Cancer, 2021, 20(1): 2.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.