Imaging Services for Cancer-Associated Fibroblasts
Cancer-associated fibroblasts (CAFs) are an essential component within the tumor microenvironment (TME). The behavior of CAFs and their interactions with other cells are of paramount importance for the development and progression of various cancers. Alfa Cytology's comprehensive and cutting-edge imaging services provide researchers with valuable insights into CAF biology, behavior, and potential therapeutic targets.
The Study of CAFs in TME
CAFs can arise from multiple sources such as resident fibroblasts, epithelial cells undergoing epithelial-to-mesenchymal transition (EMT), bone marrow-derived cells, and mesenchymal stem cells. Research has also revealed the existence of heterogeneity among CAFs, with different subtypes exhibiting unique molecular profiles and functions. Interactions between CAFs and cancer cells involve complex signaling networks, including cytokines, growth factors, extracellular matrix remodeling, and cell-cell communication mechanisms. Understanding the complex interplay between CAFs and tumor cells is crucial for developing effective cancer treatments. Advanced imaging techniques such as multiphoton microscopy, confocal microscopy, and intravital imaging allow for the visualization of CAFs in their natural environment, capturing their interactions with other cellular components in the TME. Moreover, the introduction of molecular imaging techniques has significantly improved our capability to study CAFs. Image analysis and quantification tools enable the identification and classification of different CAF subtypes based on their distinct morphological and functional characteristics. By utilizing these imaging techniques, researchers can gain valuable insights into the behavior, localization, and heterogeneity of CAFs within the TME.
Fig. 1 CAF classification scheme. (Cords L, et al., 2023)
CAF Imaging Services
Alfa Cytology offers a comprehensive range of services to explore the interactions of CAFs in the TME. Our imaging services are designed to enhance the precision and depth of CAF characterizations, providing valuable insights into their heterogeneity, infiltrative capacity, and impact on tumor growth and metastasis. Our services include, but are not limited to:
| Services | Techniques | Description |
|---|---|---|
| CAF populations isolation | Fluorescence-activated cell sorting (FACS) | Our FACS-based approach ensures the isolation of highly enriched and well-characterized CAF populations. |
| Visualization and quantification of CAFs | High-resolution immunohistochemistry (IHC) Multiplex immunofluorescence In situ hybridization |
We obtain precise identification and quantification of CAFs, thereby facilitating a comprehensive understanding of their distribution and spatial relationships within the TME. |
| Three-dimensional (3D) imaging | Confocal microscopy Multiphoton microscopy 3D reconstruction |
We can visualize the CAFs in their native three-dimensional context to assess spatial relationships, cell-cell interactions, and the structural organization of CAFs within the TME. |
| In vivo imaging | Magnetic resonance imaging (MRI) | By utilizing radiolabeled tracers that specifically target CAF markers, we are able to visualize and quantify CAF distribution and dynamics in live animal models or human samples. |
The Advantages of Our CAF Imaging Services
- We offer comprehensive services for CAF imaging, encompassing all stages from initial sample preparation to final data analysis, ensuring a seamless workflow for our clients.
- Our characterization services utilize high-resolution imaging modalities for both in vivo and in vitro studies, enabling precise investigations into the behavior of CAFs and their critical role in cancer treatment responsiveness.
- We can visualize the three-dimensional structure of CAFs, providing valuable insights into their alignment within the cellular network and facilitating a comprehensive spatial assessment.
- We utilize microfluidic platforms to mimic the TME accurately, enabling our clients to closely examine CAFs under diverse microenvironmental conditions.
At Alfa Cytology, we are dedicated to providing top-tier imaging services for CAFs. Our expertise, cutting-edge technology, and customized solutions make us the ideal partner for your CAF imaging requirements. Contact us today to discover how we can support your research and contribute to advancing our understanding of CAF biology in cancer.
Reference
- Cords L, et al. Cancer-associated fibroblast classification in single-cell and spatial proteomics data. Nat Commun, 2023, 14(1): 4294.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.