Spatial Proteome in TME

Relying on the tumor microenvironment center technology platform, Alfa Cytology provides spatial proteome detection and analysis services for TME research, helping researchers successfully achieve their research goals.


Proteins are the direct executors of life activities, so the study of protein structure, localization and protein-protein interactions will provide a direct basis for elucidating the nature of life phenomena. Space is the research level closest to the reality of tumors and microenvironments. At the spatial level, obtaining protein localization expression profiles at the spatial level of tumor tissue is of great value for the analysis of tumor tissue microenvironment, diagnosis and prognosis, and precision medicine. Because tumor spatial proteomes can understand biology including single-cell variation (at protein level and localization), dynamic protein translocation, altered interaction networks, and protein localization in multiple compartments (potentially "part-time" activities). And, the combination of global quantitative data enables the construction and systematic analysis of cellular models in the tumor microenvironment (TME) beyond qualitative descriptions.

Different approaches to imaging-based spatial proteomicsFigure 1 Different approaches to imaging-based spatial proteomics. (Lundberg E, et al., 2019)


With the popularity of spatial concepts, spatial detection technologies developed for proteomes are also competitive. At Alfa Cytology, we have established a mature tumor microenvironment center technology platform, with a group of experienced tumor microenvironment research experts and mature spatial omics technologies. We provide spatial proteome detection and analysis services for the tumor microenvironment (TME).

Service content

According to the difference of quantitative methods, Alfa Cytology provides three types of spatial proteomics technologies, namely: spatial proteomic technology based on fluorescence imaging, spatial proteomic technology based on mass spectrometry, and spatial proteomic technology based on sequencing. The several available spatial proteomic approaches are highly complementary, and their respective developmental strengths and limitations make them suitable for different types of applications. It provides a new solution for the study of tumor and microenvironment, protein interaction network and protein spatial translocation.

Spatial proteome technology service based on fluorescence imaging

Alfa Cytology provides fluorescence imaging-based spatial proteome technology services. Its core design consists of two parts: one is the detection reagent, which is composed of a primary antibody labeled with a specific oligonucleotide tag sequence (Barcode); the other is an imaging reagent, which is composed of a Barcode complementary probe labeled with a fluorophore.

Mass spectrometry-based spatial proteome technology service

Alfa Cytology uses multiple ion beam imaging (MIBI) to provide mass spectrometry-based spatial proteome technology services. Since the mass resolution of the mass spectrometer allows detection of dozens of metal tags without interference, up to 100 target proteins can be detected simultaneously.

Sequencing-based spatial proteome technology service

Alfa Cytology provides sequencing-based spatial proteome technology services using the digital spatial analysis platform (DSP). The number of targets detectable by DSP has reached 100+, covering functional proteins related to tumor, immunity and nerve.

Services of the digital spatial analysis platform (DSP) (sequencing-based spatial proteome)

DSP protein detection technology process
  • Dyeing
  • ROI selection
  • Local oligonucleotide release
  • Oligonucleotide collection
  • Quantitative
  • Data generation and analysis
Bioinformatics analysis Standard analysis
  • QC, filtering and normalization of data
  • Overview of protein expression profiles and principal component analysis (PCA)
  • AOI/ROI expression correlation analysis of samples
  • Marker expression correlation analysis of proteins
  • Differential expression analysis of proteins
  • Enrichment and differential analysis of cells in the tumor immune microenvironment
Customized analysis
The content of customized information analysis services can be negotiated and determined according to the needs of customers.

Note: Our services list and analysis content are constantly being updated and improved, please contact us by email for more up-to-date and relevant information.

Service process

Service process

Alfa Cytology is dedicated to supporting scientists in making groundbreaking scientific discoveries and developing new applications to accelerate new drug discovery and scientific diagnosis and treatment. We have established the tumor microenvironment center technology platform with high performance scientific instruments and high value solutions to ensure high quality services that enable scientists to explore the mysteries of life at the tumor microenvironment level. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. If you have any questions, please feel free to contact us.


  1. Lundberg E, Borner G H H. Spatial proteomics: a powerful discovery tool for cell biology[J]. Nature Reviews Molecular Cell Biology, 2019.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.


Alfa Cytology is a service provider specializing in tumor microenvironment research.

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