General Information
| Alternative Names | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
| Target Name | CD11b |
| Description | Rat monoclonal antibody recognizes CD11b |
| Species Reactivity | Hu, Ms, Bb, R. Mk |
| Host | Rat |
| Host Clonality | Rat mAb |
| Clone | Monoclonal |
| Clone Number | M1/70 |
| Isotope | IgG2b, kappa |
| Antigen Species | Mouse |
| Antigen | C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
| Conjugate | Un-conjugated |
| Specificity | Mouse/human/Rhesus/Baboon CD11b |
| Application | BL, FACS, ICC/IF, IHC-Fr, IP |
| Package | 100 μg |
| Form | Liquid |
| Buffer | BBS (pH 8.2) |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for IHC-Fr | Assay-dependent |
| Recommended Dilution for IF | Assay-dependent |
| Recommended Dilution for IP | Assay-dependent |
| Recommended Dilution for ICC | Assay-dependent |
| Recommended Dilution for FACS | < 1 µg/10^6 cells |
| Recommended Dilution for BL | Assay-dependent |
| Application Notes | IHC-P: PLP fixation is recommended for optimal results. Antigen Retrieval: Digested with 0.0025% Trypsin in 0.1% Calcium chloride (pH 7.8), for 5 minutes. (PMID: 7868861) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | ITGAM |
| Gene Full Name | Integrin, alpha M (complement component 3 receptor 3 subunit) |
| Background | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. |
| Biological Functions | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| Research Field | MDSC marker antibody; Myeloid-derived suppressor cell antibody |
| Predict Molecular Weight | 127 kDa |
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