General Information
| Alternative Names | Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2 |
| Target Name | CD20 |
| Description | Recombinant rabbit monoclonal antibody recognizes CD20 |
| Species Reactivity | Hu |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SQab1719 |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Synthetic peptide around the C-terminus of CD20. |
| Conjugate | Un-conjugated |
| Application | FACS, ICC/IF, IHC-P, IP, WB |
| Package | 50 μL; 100 μL |
| Form | Liquid |
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for IHC-Fr | 1:10000 ‐ 1:20000 |
| Recommended Dilution for IF | 1:400 - 1:1000 |
| Recommended Dilution for IP | 1:20 |
| Recommended Dilution for ICC | 1:400 - 1:1000 |
| Recommended Dilution for FACS | 1:100 - 1:500 |
| Application Notes | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | MS4A1 |
| Gene Full Name | Membrane-spanning 4-domains, subfamily A, member 1 |
| Background | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. |
| Biological Functions | CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt] |
| Research Field | Cancer antibody; Developmental biology antibody; Immune system antibody; B cell marker antibody; Immature B cell marker antibody; Inflammatory cell marker antibody; Tumor-infiltrating lymphocyte study antibody |
| Predict Molecular Weight | 33 kDa |
| Post-Translational Modification | Phosphorylated. Might be functionally regulated by protein kinase(s). |
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