Anti-CD3 Antibody

Online Inquiry

Catalog No.: TME-AP00017

General Information

Alternative Names	T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e
Target Name	CD3
Description	Recombinant rabbit monoclonal antibody recognizes CD3
Species Reactivity	Hu
Host	Rabbit
Host Clonality	Rabbit mAb
Clone	Monoclonal
Clone Number	SQab1713
Isotope	IgG
Antigen Species	Human
Antigen	Synthetic peptide around the N-terminus of human CD3 epsilon.
Conjugate	Un-conjugated
Specificity	This antibody has been tested not reacting to mouse spleen lysates by WB.
Application	FACS, ICC/IF, IHC-P, IP, WB
Package	50 μL; 100 μL
Form	Liquid
Purification	Purification with protein A.
Buffer	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA
Storage	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For research use only. Not intended for any clinical use.

Application Suggestions

Recommended Dilution for WB	1:1000 - 1:10000
Recommended Dilution for IHC-P	1:100 - 1:200
Recommended Dilution for IF	1:50 - 1:2000
Recommended Dilution for IP	1:15
Recommended Dilution for ICC	1:50 - 1:2000
Recommended Dilution for FACS	1:200 - 1:1000
Application Notes	IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Bioinformation

Gene Name	CD3E
Gene Full Name	CD3e molecule, epsilon (CD3-TCR complex)
Background	CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Biological Functions	CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
Research Field	Cancer antibody; Developmental biology antibody; Immune system antibody; Lymphocyte marker antibody; Inflammatory cell marker antibody; T-cell marker antibody; T-cell infiltration study antibody; Tumor-infiltrating lymphocyte study antibody
Predict Molecular Weight	23 kDa

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.