Anti-CD33 Antibody

Online Inquiry

Catalog No.: TME-AP00031

General Information

Alternative Names	p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3
Target Name	CD33
Description	Mouse monoclonal antibody recognizes CD33
Species Reactivity	Hu, NHuPrm
Host	Mouse
Host Clonality	Mouse mAb
Clone	Monoclonal
Clone Number	WM53
Isotope	IgG1
Antigen Species	Human
Antigen	Human AML cells
Conjugate	Un-conjugated
Specificity	The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Application	CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB
Package	100 μg
Form	Liquid
Purification	Purified from cell culture supernatant by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Buffer	PBS (pH 7.4) and 15 mM Sodium azide
Storage	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For research use only. Not intended for any clinical use.

Application Suggestions

Recommended Dilution for WB	Assay-dependent
Recommended Dilution for IHC-Fr	2 µg/mL
Recommended Dilution for IF	Assay-dependent
Recommended Dilution for IP	Assay-dependent
Recommended Dilution for ICC	Assay-dependent
Recommended Dilution for FACS	1 - 4 µg/mL
Recommended Dilution for CyTOF®-candidate	Assay-dependent
Application Notes	IHC-Fr: Acetone fixation. Functional application: Induction of cytokine production. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Bioinformation

Gene Name	CD33
Gene Full Name	CD33 molecule
Background	CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Biological Functions	CD33: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323). [UniProt]
Research Field	Developmental biology antibody; Immune system antibody; Human MDSC marker antibody; Myeloid-derived suppressor cell antibody
Predict Molecular Weight	40 kDa
Post-Translational Modification	Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.