General Information
| Alternative Names | CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
| Target Name | CD4 |
| Description | Recombinant rabbit monoclonal antibody recognizes CD4 |
| Species Reactivity | Hu |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SQab1714 |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Synthetic peptide around aa. 200-400 of human CD4. |
| Conjugate | Un-conjugated |
| Application | FACS, IHC-P, IP, WB |
| Package | 50 μL; 100 μL |
| Form | Liquid |
| Purification | Purification with protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for WB | 1:500 - 1:5000 |
| Recommended Dilution for IHC-Fr | 1:500 - 1:2000 |
| Recommended Dilution for IP | 1:50 |
| Recommended Dilution for FACS | 1:10 - 1:50 |
| Application Notes | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | CD4 |
| Gene Full Name | CD4 molecule |
| Background | CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. |
| Biological Functions | CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt] |
| Research Field | Developmental biology antibody; Immune system antibody; Regulatory T cells study antibody; T-cell infiltration study antibody; Tumor-infiltrating lymphocyte study antibody |
| Predict Molecular Weight | 51 kDa |
| Post-Translational Modification | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Copyright © Alfa Cytology. All rights reserved. Cookie Policy | Privacy Policy