General Information
Alternative Names | CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
Target Name | CD4 |
Description | Rabbit monoclonal antibody recognizes CD4 |
Species Reactivity | Hu |
Host | Rabbit |
Host Clonality | Rabbit mAb |
Clone | Monoclonal |
Clone Number | SP35 |
Isotope | IgG |
Antigen Species | Human |
Antigen | Synthetic peptide corresponding to internal region of human CD4. |
Conjugate | Un-conjugated |
Application | FACS, IHC-P |
Package | 50 μL; 100 μL |
Form | Liquid |
Purification | Purified by protein A/G |
Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For research use only. Not intended for any clinical use. |
Application Suggestions
Recommended Dilution for IHC-P | 1:50 |
Recommended Dilution for FACS | 1:100 (Incubate for 30 minutes at 4°C) |
Application Notes | IHC-P: Antigen Retrieval: Boil tissue section in 10mM EDTA buffer for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Positive Control | T Cell Lymphoma, Tonsil |
Bioinformation
Gene Name | CD4 |
Gene Full Name | CD4 molecule |
Background | CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. |
Biological Functions | CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt] |
Cellular Localization | Membrane |
Research Field | Developmental biology antibody; Immune system antibody; Regulatory T cells study antibody; T-cell infiltration study antibody; Tumor-infiltrating lymphocyte study antibody |
Predict Molecular Weight | 51 kDa |
Post-Translational Modification | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. |
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