General Information
| Alternative Names | Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1 |
| Target Name | CD68 |
| Description | Mouse monoclonal antibody recognizes CD68 |
| Species Reactivity | Hu, Ms, Rat, Rb |
| Host | Mouse |
| Host Clonality | Mouse mAb |
| Clone | Monoclonal |
| Clone Number | KP1 |
| Isotope | IgG1 |
| Antigen | Lysosomal contents of lung macrophages. |
| Conjugate | Un-conjugated |
| Application | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB |
| Package | 50 μL; 100 μL |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.02% Sodium azide |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for WB | Assay-dependent |
| Recommended Dilution for IHC-FoFr | Assay-dependent |
| Recommended Dilution for IHC-Fr | 1:200 - 1:400 |
| Recommended Dilution for IHC-P | 1:200 - 1:400 |
| Recommended Dilution for IF | Assay-dependent |
| Recommended Dilution for ICC | Assay-dependent |
| Recommended Dilution for FACS | 1:100 - 1:1000 for 10^6 cells in 100 μL |
| Application Notes | IHC-P: Antigen Retrieval: Boil tissue section in Tris-EDTA pH9.0 or Citrate buffer (pH 6.0). Incubate with antibody at 4°C for overnight. IHC-Fr: Incubate with the antibody at 4°C for overnight or at RT for 30-60 mins. Flow cytometry: Membrane permeabilisation is required for this application. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | CD68 |
| Gene Full Name | CD68 molecule |
| Background | CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. |
| Biological Functions | CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt] |
| Research Field | Immune system antibody; Activated macrophage/microglia study antibody; Neuroinflammation study antibody; Active macroglial marker antibody; M1/M2/TAM marker antibody; Macrophage marker antibody; M1 macrophage marker antibody; Inflammatory cell marker antibody |
| Predict Molecular Weight | 37 kDa |
| Post-Translational Modification | N- and O-glycosylated. |
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