General Information
| Alternative Names | T-cell surface glycoprotein CD8 alpha chain; Leu2; p32; T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; CD antigen CD8a |
| Target Name | CD8 |
| Description | Recombinant rabbit monoclonal antibody recognizes CD8 |
| Species Reactivity | Hu |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SQab19146 |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Synthetic peptide within aa. 135-235 of human CD8. |
| Conjugate | Un-conjugated |
| Application | IHC-P |
| Package | 100 μL |
| Form | Liquid |
| Purification | Purification with protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for IHC-P | 1:100 - 1:200 |
| Application Notes | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | CD8A |
| Gene Full Name | CD8a molecule |
| Background | CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. |
| Biological Functions | CD8 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: CD8A localizes to lipid rafts only when associated with its partner CD8B. Isoform 2: Secreted. [UniProt] |
| Research Field | Developmental biology antibody; Immune system antibody; Cytotoxic T antibody; Cytotoxic T Cell surface study antibody; Tumor-infiltrating lymphocyte study antibody |
| Predict Molecular Weight | 26 kDa |
| Post-Translational Modification | All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. [UniProt] |
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