General Information
| Alternative Names | Fibroblast activation protein alpha; SIMP; Seprase; Integral membrane serine protease; Surface-expressed protease; EC 3.4.21.26; Dipeptidyl peptidase FAP; 170 kDa melanoma membrane-bound gelatinase; EC 3.4.14.5; APCE; Serine integral membrane protease; DPPIV; EC 3.4.21.-; FAPalpha; Gelatine degradation protease FAP; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; FAPA |
| Target Name | FAP / Fibroblast activation protein |
| Description | Rabbit polyclonal antibody recognizes FAP / Fibroblast activation protein |
| Species Reactivity | Hu, Ms, Rat |
| Host | Rabbit |
| Host Clonality | Rabbit pAb |
| Clone | Polyclonal |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | KLH-conjugated synthetic peptide within the center region of human FAP / Fibroblast activation protein. |
| Conjugate | Un-conjugated |
| Application | ICC/IF, IHC-P, WB |
| Package | 100 μL |
| Form | Liquid |
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for WB | 1:500 - 1:1000 |
| Recommended Dilution for IHC-P | 1:100 - 1:200 |
| Recommended Dilution for IF | 1:100 - 1:500 |
| Recommended Dilution for ICC | 1:100 - 1:500 |
| Application Notes | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | FAP |
| Gene Full Name | Fibroblast activation protein, alpha |
| Background | The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. |
| Biological Functions | Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner. [UniProt] |
| Cellular Localization | Plasma membrane. Note: Localized on cell surface with lamellipodia and invadopodia membranes and on shed vesicles. Secreted. Note: Found in blood plasma and serum. [UniProt] |
| Predict Molecular Weight | 88 kDa |
| Post-Translational Modification | N-glycosylated. The N-terminus may be blocked. [UniProt] |
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