General Information
| Alternative Names | XPID; AIID; JM2; IPEX; PIDX; Forkhead box protein P3; Scurfin; DIETER |
| Target Name | FOXP3 |
| Description | Rabbit monoclonal antibody recognizes FOXP3 |
| Species Reactivity | Hu |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SP97 |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Synthetic peptide corresponding to C-terminus of human FOXP3 protein. |
| Conjugate | Un-conjugated |
| Application | IHC-P |
| Package | 50 μL; 100 μL |
| Form | Liquid |
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for IHC-P | 1:100 |
| Application Notes | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubation Time: 20 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| Positive Control | Tonsil |
Bioinformation
| Gene Name | FOXP3 |
| Gene Full Name | Forkhead box P3 |
| Background | FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at a low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells. |
| Cellular Localization | Nucleus |
| Research Field | Cell biology and cellular response antibody; Gene regulation antibody; Immune system antibody; Regulatory T cells study antibody |
| Predict Molecular Weight | 47 kDa |
| Post-Translational Modification | Polyubiquitinated, leading to its proteasomal degradation in regulatory T-cells (Treg) which is mediated by STUB1 in a HSPA1A/B-dependent manner. Deubiquitinated by USP7 leading to increase in protein stability. Phosphorylation at Ser-418 regulates its transcriptional repressor activity and consequently, regulatory T-cells (Treg) suppressive function. Dephosphorylated at Ser-418 by protein phosphatase 1 (PP1) in Treg cells derived from patients with rheumatoid arthritis. Phosphorylation by CDK2 negatively regulates its transcriptional activity and protein stability (By similarity). Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1. Undergoes proteolytic cleavage in activated regulatory T-cells (Treg), and can be cleaved at either the N- or C-terminal site, or at both sites. |
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