General Information
| Alternative Names | HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39 |
| Target Name | iNOS |
| Description | Rabbit polyclonal antibody recognizes iNOS |
| Species Reactivity | Hu, Ms, Rat, Mamm |
| Host | Rabbit |
| Host Clonality | Rabbit pAb |
| Clone | Polyclonal |
| Isotope | IgG |
| Antigen Species | Mouse |
| Antigen | Purified enzyme from mouse macrophages (RAW 264.7) cells. |
| Conjugate | Un-conjugated |
| Specificity | This antibody may cross-react to nNOS (~5%) and does not cross-react to eNOS. |
| Application | ICC/IF, IHC-P, IP, WB |
| Package | 250 μL |
| Form | Liquid |
| Purification | Purification with protein A. |
| Buffer | TBS (pH 7.4) and 0.5 mg/mL BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for WB | 1:200 - 1:1000 |
| Recommended Dilution for IHC-P | 1:200 - 1:1000 |
| Recommended Dilution for IF | 1:200 |
| Recommended Dilution for IP | 1:200 |
| Recommended Dilution for ICC | 1:200 |
| Application Notes | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 or Tris-EDTA pH 9.0 for 20 min followed by cooling at RT for 20 min. Incubate with the antibody at 4°C for overnight. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | Nos2 |
| Gene Full Name | Nitric oxide synthase 2, inducible |
| Background | iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. |
| Biological Functions | iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt] |
| Research Field | Inflammation study antibody; M1/M2/TAM marker antibody; Macrophage marker antibody; M1 macrophage marker antibody |
| Predict Molecular Weight | 131 kDa |
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