General Information
| Alternative Names | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
| Target Name | Ki-67 |
| Description | Recombinant rabbit monoclonal antibody recognizes Ki-67 |
| Species Reactivity | Hu |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SQab1897 |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Synthetic peptide within aa. 1000-1100 of human Ki-67. |
| Conjugate | Un-conjugated |
| Application | IHC-P |
| Package | 100 μL |
| Form | Liquid |
| Purification | Purification with protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for IHC-P | 1:100 - 1:200 |
| Application Notes | IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | MKI67 |
| Gene Full Name | Marker of proliferation Ki-67 |
| Background | Ki-67 is a nuclear protein. It is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. |
| Biological Functions | Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| Research Field | Microvascular density study antibody; Proliferation marker antibody |
| Predict Molecular Weight | 359 kDa |
| Post-Translational Modification | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. [UniProt] |
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