General Information
| Alternative Names | Gr1; Gr-1; Ly-6G |
| Target Name | Ly6G + Ly6C |
| Description | Rat monoclonal antibody recognizes Ly6G + Ly6C |
| Species Reactivity | Ms |
| Host | Rat |
| Host Clonality | Rat mAb |
| Clone | Monoclonal |
| Clone Number | RB6-8C5 |
| Isotope | IgG2b, kappa |
| Antigen Species | Mouse |
| Conjugate | Un-conjugated |
| Specificity | This antibody recognizes the mouse Gr-1 antigen, a ~21 - 25 kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins. |
| Application | Depletion, FACS, IHC-Fr, IHC-P, IP |
| Package | 100 μg |
| Form | Liquid |
| Buffer | BBS (pH 8.2) |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for IHC-Fr | Assay-dependent |
| Recommended Dilution for IHC-P | Assay-dependent |
| Recommended Dilution for IP | Assay-dependent |
| Recommended Dilution for FACS | 1:50 - 1:100 (Use 10 µL to label 10^6 cells in 100 µL) |
| Recommended Dilution for Depletion | Assay-dependent |
| Application Notes | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | Ly6g |
| Gene Full Name | Lymphocyte antigen 6 complex, locus G |
| Background | Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. |
| Research Field | Mouse inflammatory cell marker antibody; Neurophil marker antibody; Mouse MDSC marker antibody; Myeloid-derived suppressor cell antibody |
| Predict Molecular Weight | 12 kDa |
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