General Information
| Alternative Names | Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325 |
| Target Name | N-cadherin |
| Description | Rabbit polyclonal antibody recognizes N-cadherin |
| Species Reactivity | Hu, Ms, Rat, Sheep |
| Host | Rabbit |
| Host Clonality | Rabbit pAb |
| Clone | Polyclonal |
| Isotope | IgG |
| Antigen Species | Human |
| Antigen | Carrier protein coupled synthetic peptide corresponding to aa. 811-824 of human N cadherin. |
| Conjugate | Un-conjugated |
| Specificity | The antibody detects a band at ~ 130 kDa in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with E-cadherin. |
| Application | ELISA, FACS, ICC/IF, IHC-P, IP, WB |
| Package | 50 μL |
| Form | Liquid |
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/mL BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for ELISA | 1:2000 |
| Recommended Dilution for WB | 1:1000 |
| Recommended Dilution for IHC-P | 1 µg/mL |
| Recommended Dilution for IF | 1:200 |
| Recommended Dilution for IP | 1:100 |
| Recommended Dilution for ICC | 1:200 |
| Recommended Dilution for FACS | 1:100 |
| Application Notes | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | CDH2 |
| Gene Full Name | Cadherin 2, type 1, N-cadherin (neuronal) |
| Background | N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. |
| Biological Functions | N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell membrane, sarcolemma. Cell junction. Cell surface. Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt] |
| Research Field | EMT study antibody; Mesenchymal markers antibody |
| Predict Molecular Weight | 100 kDa |
| Post-Translational Modification | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity). May be phosphorylated by OBSCN. [UniProt] |
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