General Information
| Alternative Names | Vimentin; CTRCT30; HEL113 |
| Target Name | Vimentin |
| Description | Recombinant rabbit monoclonal antibody recognizes vimentin |
| Species Reactivity | Hu, Ms |
| Host | Rabbit |
| Host Clonality | Rabbit mAb |
| Clone | Monoclonal |
| Clone Number | SQab1721 |
| Antigen Species | Human |
| Antigen | Synthetic peptide around the C-terminus of vimentin |
| Conjugate | Un-conjugated |
| Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Package | 50 μL; 100 μL |
| Form | Liquid |
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA |
| Storage | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For research use only. Not intended for any clinical use. |
Application Suggestions
| Recommended Dilution for WB | 1:1000 - 1:2000 |
| Recommended Dilution for IHC-Fr | Assay-dependent |
| Recommended Dilution for IHC-P | 1:500 - 1:1000 |
| Recommended Dilution for IF | 1:10000 - 1:25000 |
| Recommended Dilution for IP | 1:50 |
| Recommended Dilution for ICC | 1:10000 - 1:25000 |
| Recommended Dilution for FACS | 1:100 - 1:500 |
| Application Notes | IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 IHC-Fr: guaranteed based on publication PMID: 35011683 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Bioinformation
| Gene Name | VIM |
| Gene Full Name | Vimentin |
| Background | Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. |
| Biological Functions | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt] |
| Research Field | Cancer antibody; Controls and markers antibody; Developmental biology antibody; Neuroscience antibody; Signaling transduction antibody; Cancer-associated fibroblast antibody; CAF marker antibody; EMT study antibody; Mesenchymal markers antibody; Fibroblast marker antibody; Muller Cell marker antibody; Sarcoma marker antibody |
| Predict Molecular Weight | 54 kDa |
| Post-Translational Modification | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
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