Catalog No.: TME-PT01726
Product Details
| Target Name | Integrin alpha 10 beta 1 |
| Alternative Names | Integrin alpha 10 beta 1, ITGA10&ITGB1 |
| Size | 100 μg, 1 mg |
| Species | Mouse |
| Tag | His Tag, Tag Free |
| Host | Human 293 cells (HEK293) |
| Conjugate | Unconjugated |
| Sequence | AA Phe 23 - Thr 1118 (ITGA10) & Gln 21 - Asp 728 (ITGB1) |
| Molecular Characterization | The protein we provide is produced by co-expression of ITGA10 and ITGB1, with a calculated MW of 126.6 kDa (ITGA10) and 83.5 kDa (ITGB1). Subunit ITGA10 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 150-170 kDa (ITGA10) and 100-130 kDa (ITGB1) respectively due to glycosylation. |
| Purity | >90% as determined by SDS-PAGE. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization. |
| Storage | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. |
| Endotoxin | Less than 1.0 EU per μg by the LAL method. |
| Background | The recombinant form of human integrin alpha(10)I domain was used to investigate its binding specificity. It was observed that alpha(10)I recognized collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner. However, its binding to tenascin was only slightly better than its binding to albumin. Alpha 10 beta 1 is an integrin known to bind collagen, along with other integrins such as α1β1, α2β1, and α11β1. Interestingly, the GROGER motif found in the N-terminal domain of collagens I and III was weakly recognized by α10β1, which is an important collagen receptor on chondrocytes. This behavior contrasts with the other collagen-binding integrins. |
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